Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Genes defining the CD14+ single-cell clusters and variance across patient samples.
  • Fig. S2. Identification of synovial HBEGF+ inflammatory macrophages in an independent RA patient study.
  • Fig. S3. Synovial fibroblasts down-regulate several pathways in TNF-induced macrophages and impose a transcriptome consistent with TNF and prostaglandin exposure.
  • Fig. S4. Synovial fibroblasts express EGF receptors whereas HBEGF+ inflammatory macrophages express two EGF ligands.
  • Fig. S5. Synovial fibroblast exposure modifies how RA medications affect TNF-induced macrophages.
  • Table S1. Patient characteristics for CD14+ synovial single-cell samples.
  • Table S2. Patient characteristics for CD14+ synovial bulk-sorted samples.
  • Table S3. RA patient characteristics for the ex vivo synovial tissue assay.
  • Table S4. Individual patient disease diagnosis, serology, and histology for the ex vivo synovial tissue assay.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Individual subject-level data.