Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Cardiac function of CH and normoxic rats at baseline.
  • Fig. S2. Schematic diagram of rat CPB model.
  • Fig. S3. CIRBP expression and postoperative cardiac function in male and female CH rats.
  • Fig. S4. Circos plot showing global methylation patterns and locations of DMRs in different chromosomes.
  • Fig. S5. Genome-wide DNA methylation analysis of cardiac tissues from CH rats.
  • Fig. S6. Transcriptomic analysis of cardiac tissues from CH rats.
  • Fig. S7. The methylation degree of CpG island in Cirbp was evaluated by high-throughput bisulfite sequencing.
  • Fig. S8. 5-Aza restored CIRBP expression and its response to hypothermia.
  • Fig. S9. Generation of Cirbp−/− and Cirbp-Tg rats.
  • Fig. S10. Baseline assessment of organ histology and cardiac function of wild-type, Cirbp−/−, and Cirbp-Tg rats.
  • Fig. S11. Cirbp knockout in CH rats further attenuates hypothermic cardioprotection during CPB and overexpression of CIRBP reverses this process.
  • Fig. S12. Cardiac-specific knockdown of COQ6 or COQ9 in wild-type rats attenuates hypothermic cardioprotection during CPB.
  • Fig. S13. Cardiac-specific overexpression of COQ6 or COQ9 in Cirbp−/− rats partially reverses impaired hypothermic cardioprotection during CPB.
  • Fig. S14. CoQ10 addition in cardioplegic solution during CPB significantly improves cardioprotection in Cirbp−/− rats.
  • Fig. S15. CH impaired hypothermia-induced cardiac CoQ10 biosynthesis during CPB.
  • Fig. S16. Schematic summarizing mechanism.
  • Table S1. Arterial blood gas analysis in the animals at the end of a 4-week exposure to a normoxic or hypoxic environment.
  • Table S2. Significantly different proteins (fold change < 0.83 or fold change > 1.2; P < 0.05) in cardiac tissues from CH rats versus normoxic rats.
  • Table S3. Baseline characteristics of patients.
  • Table S4. Significantly differently expressed proteins (fold change < 0.83 or fold change > 1.2; P < 0.05) in cardiac tissues from Cirbp−/− rats versus wild-type rats.
  • Table S5. Primer sequences used in the high-throughput bisulfite sequencing.
  • Table S6. Primer sequences used in bisulfite sequencing PCR combined with TA cloning.
  • Table S7. Primer pairs used in the biotin pull-down assay.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Primary data.
  • Movie S1 (.mp4 format). Movie of rat CPB model.