Supplementary Materials

The PDF file includes:

  • Fig. S1. R26mTmG gene locus of interest and lack of gene editing in nonpulmonary organs after intra-amniotic delivery.
  • Fig. S2. Distribution of gene-edited cells in the lung.
  • Fig. S3. Gene editing in pulmonary cell types.
  • Fig. S4. Selection of sgRNAs for excision of the Sftpc gene and in vivo gene editing in C57BL/6 and SftpcI73T/WT mice.
  • Fig. S5. Transmission electron microscopy of gene-edited lungs of SftpcI73T/WT mice.
  • Fig. S6. Lung morphology of gene-edited SftpcI73/WT mice in adulthood.
  • Table S1. mTmg and Sftpc sgRNAs for in vitro and in vivo editing.
  • Table S2. Primers used for surveyor assay for Sftpc sgRNAs.
  • Table S3. Primers used for PCR and Sanger sequencing.
  • Table S4. Primers used for qPCR for Sftpc gene deletion.
  • Table S5. Primer sequences for NGS of Sftpc off-target sites.
  • Table S6. Analysis of 20 off-target sites.

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Intra-amniotic injection of dilute trypan blue at E16.
  • Movie S2 (.mp4 format). In utero gene-edited SftpcI73T/WT mouse at P7.
  • Data file S1 (Microsoft Excel format). Original data.