Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Experimental validation of the FTO-entacapone interaction and entacapone target selectivity assessment.
  • Fig. S2. Crystal complex structures of FTO bound with different types of inhibitors.
  • Fig. S3. Dose-response effect of entacapone on the body weight of DIO mice.
  • Fig. S4. Generation and phenotypic measurements of liver-specific Fto knockout mice.
  • Fig. S5. Hepatic Fto regulates gluconeogenesis in the liver.
  • Fig. S6. FTO-FOXO1 regulatory axis modulates G6PC and PCK1 expression in a hepatic cell line.
  • Fig. S7. Hepatic Fto knockdown in db/db mice and enzymatic activity of FTO R96A mutant protein.
  • Fig. S8. Characterization of m6A modifications and changes in gene expression in mouse adipose tissues.
  • Fig. S9. Generation, RNA-seq analysis, and phenotypic analysis of Foxo1-Mut mice with the Foxo1 synonymous mutation at the Foxo1–m6A-2 site.
  • Fig. S10. Proposed FTO-FOXO1 regulatory axis in adipose tissue and liver.
  • Table S1. Statistics on data collection and structure refinement of FTO-inhibitor complex structures.
  • Table S2. Structures and FTO enzymatic inhibition activities of 19 selected compounds from virtual screening.
  • References (6278)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Summary of all reported data values.