Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Phenotypic differences between pre-outbreak and outbreak E. faecalis strains.
  • Fig. S2. Comparison of E. faecalis B594 and V583 strain genomes.
  • Fig. S3. Phylogeny of 62 E. faecalis outbreak strains.
  • Fig. S4. Summary of gene expression differences between gntR wild-type and mutant E. faecalis strains measured by RNA sequencing.
  • Fig. S5. Difference in autolysis between gntR wild-type and mutant strains.
  • Fig. S6. Morphological differences between gntR wild-type and mutant strains.
  • Fig. S7. Growth curves of MMH594 wild-type and mutant strains in the presence of different sugars.
  • Fig. S8. Active site modeling of the GHF65 enzyme.
  • Fig. S9. Increased resistance to ampicillin killing in gntR mutants due to the constitutive expression of the GHF65 enzyme.
  • Fig. S10. Killing assay in whole human blood with pbp4 wild-type and mutant strains.
  • Fig. S11. Predicted secondary structure of the region upstream of pbp4 and location of mutations identified in in vitro–selected meropenem-resistant strains.
  • References (4472)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Strain and genome information for analyzed strains.
  • Table S2 (Microsoft Excel format). Gene enrichment among outbreak lineages.
  • Table S3 (Microsoft Excel format). Filtered variants detected in 62 outbreak strains.
  • Table S4 (Microsoft Excel format). Variants detected in outbreak strains isolated from the same patient.
  • Table S5 (Microsoft Excel format). GntR and PBP4 mutations identified in nonoutbreak strains.