Supplementary Materials

The PDF file includes:

• Materials and Methods
• Fig. S1. Scheme of in vivo molecular MRI of renal fibrosis in three independent mouse models of renal fibrosis.
• Fig. S2. Confirmation of increased elastin expression in renal fibrosis.
• Fig. S3. Elastin expression in murine models of renal fibrosis.
• Fig. S4. Identification of interstitial (myo-)fibroblasts as elastin-producing cells in fibrotic kidneys.
• Fig. S5. Resident fibroblasts express elastin.
• Fig. S6. Elastin expression in CKD patients.
• Fig. S7. Staining validation by means of nonspecific secondary antibody and buffer control.
• Fig. S8. Feasibility assessment of different MRI sequences and measurement times.
• Fig. S9. Elastin imaging detects fibrosis in adenine nephropathy.
• Fig. S10. Elastin imaging detects renal fibrosis in I/R.
• Fig. S11. Elastin imaging detects renal fibrosis in UUO.
• Fig. S12. Molecular MRI captures therapy effects in renal fibrosis.
• Fig. S13. Molecular MRI analysis of renal fibrosis versus routine measurement of kidney function.
• Fig. S14. Overall study and experimental design.
• Table S1. Collection of patient fibrotic kidney samples reflecting different kidney diseases.
• Table S2. IgA nephropathy patient cohort.
• Table S3. List of primers used for qRT-PCR.
• References (38–40)

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

• Data file S1 (Microsoft Excel format). Individual subject-level data.