Supplementary Materials

The PDF file includes:

  • Fig. S1. Existence of GLY1 in mutant PANX1 and effect of mutations on cellular localization of PANX1 in HeLa cells.
  • Fig. S2. Effects of R217H and I272V on oocyte survival and effects of C347S and 21_23delTEP on zygote survival.
  • Fig. S3. Association of oocyte death phenotype with mutant GLY1 bands.
  • Fig. S4. Decrease of the maximum current by application of CBX measured at +60 mV.
  • Fig. S5. Measurement of intracellular ATP content in oocytes or zygotes injected with WT or mutant PANX1 cRNA.
  • Fig. S6. Scheme for generation of human mutant PANX1 KI mice and evaluation of their fertility.
  • Fig. S7. Comparison of PANX1 expression in human and mouse oocytes.
  • Fig. S8. Schematic strategy for targeted integration of human PANX1 gene at the mouse ROSA26 locus.
  • Fig. S9. Effect of PNGase F and Endo H treatment on PANX1 glycosylation in oocytes from OE- PANX1WT and OE-PANX1Q392* mice.
  • Fig. S10. Intracellular ATP measurement in oocytes from OE-PANX1WT and OE-PANX1Q392* mice and the effect of probenecid on OE-PANX1Q392* oocyte maturation.
  • Table S1. Primers used for PANX1 exon sequencing, mouse genotyping, and qRT-PCR.
  • Table S2. Numbers of oocytes used in Fig. 4 (C, D, and F).

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Other Supplementary Material for this manuscript includes the following: