Supplementary Materials

The PDF file includes:

  • Fig. S1. Creation of transgenic mice expressing human BIN1 (TgBIN1 mouse).
  • Fig. S2. Increased BIN1 expression rescues the motor function and muscle force of Mtm1−/y mice.
  • Fig. S3. Increased BIN1 expression rescues muscle histology of Mtm1−/y mice at 2 and 7 months old.
  • Fig. S4. Postnatal intramuscular overexpression of BIN1 rescues muscle force and myofiber organization in Mtm1−/y mice.
  • Fig. S5. Organ weights and muscle fatigue in the Mtm1−/y mice expressing BIN1 after systemic AAV delivery.
  • Fig. S6. Extracellular matrix defects in Mtm1−/y muscle.
  • Fig. S7. Focal adhesion defects in Mtm1−/y myofibers.
  • Table S1. Breeding strategy and outcome for Mtm1−/y × Bin1−/+ with expected mice and obtained at E18.5 and 10 days after birth.
  • Table S2. Breeding strategy and outcome for Bin1−/+ × Bin1−/+ TgBIN1 with expected mice and obtained at E18.5 and 10 days after birth.
  • Legend for table S3
  • Legends for movies S1 to S3

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Other Supplementary Material for this manuscript includes the following:

  • Table S3. Raw data (Excel file).
  • Movie S1 (.mp4 format). Expression of human BIN1 did not generate any obvious clinical phenotypes in mice.
  • Movie S2 (.mp4 format). Increased BIN1 expression rescues Mtm1−/y phenotype.
  • Movie S3 (.mp4 format). Postnatal systemic injection of AAV-BIN1 rescued Mtm1−/y mice phenotypes.