Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Determination of the Kd for the dimeric protein complexes.
  • Fig. S2. Penetration of the Omomyc mini-protein into human cancer cells besides NCSLC.
  • Fig. S3. Impairment of the cell entrance of Omomyc in A549 cells by mutation of three arginine residues in its DNA binding domain.
  • Fig. S4. MYC status in the cell lines used in this study and sensitivity to MYC siRNA.
  • Fig. S5. MYC-related gene sets affected by Omomyc treatment in H1299 cells.
  • Fig. S6. MYC-related gene sets affected by Omomyc treatment in H1975 cells.
  • Fig. S7. MYC-related gene sets affected by Omomyc treatment in A549 cells.
  • Fig. S8. H3K27Ac ChIP-qPCR analysis and MYC ChIP-seq analysis of MYC sequence motif and super-enhancers.
  • Fig. S9. Specificity of the anti-Omomyc polyclonal antibody used in this study.
  • Fig. S10. MYC-related gene sets affected by 3 days of Omomyc treatment in tumors from KRasLSL-G12D/+-driven lung adenocarcinoma mouse model.
  • Fig. S11. Effect of the direct pulmonary administration of the mini-protein on mouse weight and tumor growth.
  • Fig. S12. Pharmacokinetic analysis of Omomyc-DFO-89Zr (2.62 mg/kg) administered intravenously to healthy mice.
  • Fig. S13. Safety analysis of the systemic administration of the Omomyc mini-protein, as monotherapy or in combination with paclitaxel.
  • References (6468)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 contains the following supplementary tables (Excel file):
  • Table S1 (Microsoft Excel format). Van’t Hoff analysis of the thermal denaturation curves.
  • Table S2 (Microsoft Excel format). Analysis of the titration of the E-box with Omomyc and c-Myc°.
  • Table S3 (Microsoft Excel format). GSEA comparing gene expression of vehicle-treated versus Omomyc-treated cells.
  • Table S4 (Microsoft Excel format). Differential gene expression from the microarray analysis of H1975 cells.
  • Table S5 (Microsoft Excel format). Differential gene expression from the microarray analysis of H1299 cells.
  • Table S6 (Microsoft Excel format). Differential gene expression from the microarray analysis of A549 cells.
  • Table S7 (Microsoft Excel format). Differential gene expression from the microarray analysis of lung tumors from mice treated for 3 days by intranasal administration.
  • Table S8 (Microsoft Excel format). Primer sequences used for the ChIP-qPCR experiments.
  • Data file S2 contains the original data (Excel file).
  • Movie S1 (.mp4 format). Animated 3D rendering of mPET/mCT imaging of lungs of a tumor-bearing mouse 24 hours after intranasal administration of Omomyc-DFO-89Zr (2.37 mg/kg).