Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Epithelial tumor cells exhibit greater MAPK signaling.
  • Fig. S2. MAPK signaling in epithelial cells is only partially dependent on FAK-PAK signaling.
  • Fig. S3. IL17RD expression in epithelial cancer cells correlates with MAPK signaling and inversely correlates with FGFR1.
  • Fig. S4. ZEB1-expressing lung cancer cells are resistant to MEK inhibition.
  • Fig. S5. MiR-200 sensitizes mesenchymal lung tumors to MEK inhibition through IL17RD up-regulation.
  • Fig. S6. ZEB1 expression correlates with decreased IL17RD and decreased MAPK signaling in Kras mutant mouse lung tumors.
  • Fig. S7. KrasG12D;miR-141/200c−/+ (KM) mouse lung tumors display high ZEB1, low MAPK signaling, and heterogeneity.
  • Fig. S8. ZEB1 expression in MEK inhibitor–treated lung tumors of Kras mutant mice correlates with resistance.
  • Fig. S9. Mocetinostat recapitulates miR-200 expression, sensitizing resistant tumor cells to MEK inhibition.
  • Fig. S10. ZEB1 inversely correlates with ERK phosphorylation in patients with lung ADC.
  • Legends for tables S1 to S16

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 contains the following supplementary tables:
  • Table S1 (Microsoft Excel format). Quality control metrics for the shRNA screens.
  • Table S2 (Microsoft Excel format). Gene-level dropout scores for each of the four screens described in Fig. 1A.
  • Table S3 (Microsoft Excel format). RPPA dataset of protein expression fold change between epithelial and mesenchymal human lung cancer cell lines.
  • Table S4 (Microsoft Excel format). RPPA dataset of protein expression fold change between epithelial and mesenchymal murine lung cancer cell lines.
  • Table S5 (Microsoft Excel format). RPPA dataset of protein expression fold change in mesenchymal H157 cells after induced miR-200 expression.
  • Table S6 (Microsoft Excel format). RPPA dataset of protein expression fold change in mesenchymal H1299 cells after induced miR-200 expression.
  • Table S7 (Microsoft Excel format). RPPA dataset of protein expression fold change in epithelial HCC827 cells with constitutive ZEB1 expression.
  • Table S8 (Microsoft Excel format). RPPA dataset of protein expression fold change in epithelial H441 cells after induced ZEB1 expression.
  • Table S9 (Microsoft Excel format). Individual mouse subcutaneous tumor volume measurements from experiment in Fig. 4D.
  • Table S10 (Microsoft Excel format). Individual mouse subcutaneous tumor volume measurements from experiment in Fig. 5D.
  • Table S11 (Microsoft Excel format). Individual mouse subcutaneous tumor volume measurements from experiment in Fig. 5E.
  • Table S12 (Microsoft Excel format). Individual mouse subcutaneous tumor volume measurements from experiment in Fig. 5F.
  • Table S13 (Microsoft Excel format). Individual mouse lung tumor area measurements and percent change in lung tumor area from experiment in Fig. 6B.
  • Table S14 (Microsoft Excel format). Individual mouse subcutaneous tumor volume measurements from experiment in Fig. 7F.
  • Table S15 (Microsoft Excel format). Individual mouse lung tumor area measurements and percent change in lung tumor area from experiment in Fig. 7G.
  • Table S16 (Microsoft Excel format). All commercially used antibodies, primers, shRNA, and complementary DNA open reading frames, including catalog numbers and sequences.