Supplementary Materials

The PDF file includes:

  • Fig. S1. Analysis of CAR expression at the cell surface.
  • Fig. S2. Transient depletion of CD19+ B cells from one NZB/W mouse.
  • Fig. S3. Immunofluorescence images of representative glomeruli from CAR T cell–treated and control MRL-lpr mice.
  • Fig. S4. Comparison of alopecia and skin lesions between CAR T cell–treated and negative control MRL-lpr mice.
  • Fig. S5. Analysis of CAR T cell cytotoxicity in vivo.
  • Fig. S6. qPCR of transcripts expressed in sorted CD8+ T cells.
  • Fig. S7. Reverse transcription qPCR of CAR RNA in bone marrow and spleen of CD19+ depleted and CD19+ intact NZB/W mice.
  • Fig. S8. Typical gating strategy.
  • Table S1. Comparison of viral vectors used to transduce T cells.
  • Table S2. List of oligonucleotide primers used for RT-PCR.

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Other Supplementary Material for this manuscript includes the following:

  • Table S3 (Microsoft Excel format). CD4+ T cell subsets.
  • Data file S1 (Microsoft Excel format). Primary data.