Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. The viability of human T cells is preserved over a wide range of NaCl concentrations.
  • Fig. S2. TH17 cell signature molecules are up-regulated upon stimulation of memory TH cells with NaCl.
  • Fig. S3. NaCl increases memory TH2 cell responses independently of exogenous or autocrine IL-4.
  • Fig. S4. NaCl induces up-regulation of IL-4, IL-17, as well as IL-4 and IL-17 double-positive T cells.
  • Fig. S5. NaCl enhances TH2 and suppresses TH1 cell responses in TH cell clones.
  • Fig. S6. Comparison of different osmolytes demonstrates NaCl to be the most potent inducer of IL-4 and suppressor of IFN-γ in memory T cells.
  • Fig. S7. Memory CD8 T cells show stable IL-4 and IFN-γ expression upon stimulation with NaCl but up-regulate IL-17.
  • Fig. S8. Skin T cells are distinct from blood T cells and differ in their expression of the tissue residency markers CD69 and CD103.
  • Fig. S9. NaCl induces the up-regulation of the skin-homing chemokine receptor CCR8, which enriches for TH2-associated cytokines.
  • Fig. S10. Transcriptome-wide enrichment of a TH2 cell signature by NaCl.
  • Fig. S11. NaCl induces TH2 cell polarization independently of exogenous or autocrine IL-4.
  • Fig. S12. TH17-polarizing cytokine conditions abrogate the TH2-promoting effect of NaCl.
  • Fig. S13. Mouse T cells differentiate into TH2 cells in response to NaCl.
  • Fig. S14. SGK-1 is a downstream target of NFAT5.
  • Fig. S15. GATA-3 and T-bet are not regulated by NFAT5 and SGK-1 in low NaCl conditions.
  • Fig. S16. Model.
  • Table S1. Selected regions and primer sequences used for DNA methylation analyses.
  • Table S2. Patient information.
  • References (5054)

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