Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Phenotypic characterization of tumor-infiltrating MMLCs.
  • Fig. S2. Production of indicated monocyte chemotactic proteins by tumor, distant lung tissue dissociates, and PBMCs.
  • Fig. S3. HLA-DRhiCD14+ TAM coexpress M1/M2 markers, as well as T cell coinhibitory and costimulatory receptors.
  • Fig. S4. The accumulation of nonclassical CD14intCD16hi monocytes in tumor, distant lung tissue, and blood.
  • Fig. S5. Production of factors involved in monocyte recruitment and macrophage differentiation/polarization in tumor, distant lung dissociates, and blood.
  • Fig. S6. The expression of T cell coinhibitory and costimulatory receptors on MMLCs.
  • Fig. S7. Effects of tumor CD14+ cells on NY-ESO-1–specific T cell responses.
  • Fig. S8. The expression of key T cell suppressive genes in CD14+ MMLCs and their correlation with the ability of tumor MMLCs to regulate tumor-specific T cell responses.
  • Fig. S9. Production of key T cell suppressive factors by CD14+ MMLCs and their correlation with the ability of tumor MMLCs to regulate tumor-specific T cell responses.
  • Fig. S10. Common T cell suppressive mechanisms in the regulation of tumor-specific Ly95 response by early-stage tumor MMLCs.
  • Fig. S11. Role of TAM-derived PD-L1 in the regulation of tumor-specific T cell responses.
  • Fig. S12. Cross-presentation of NY-ESO-1 by TAMs and role of TAM-expressed PD-L1 in the regulation of cytotoxic activity of Ly95 cells.
  • Fig. S13. Correlation analysis of the presence of MMLCs in lung tumor with overall survival.
  • Fig. S14. Correlation analysis of the accumulation of MMLC populations with the frequency and function of tumor-associated neutrophils, Tregs, and CD8 cells in tumor.
  • Fig. S15. Correlation analysis of the ability of tumor CD14+ cells to regulate T cell responses with accumulation of CD8+ T cells, Tregs, and IFN-γ production by CD8+ T cells in tumor.
  • Table S1. Patient characteristics.
  • Table S2. Correlation analysis of the phenotypic and functional characteristics of tumor CD14+ cells with clinical parameters of patients with LC.
  • References (42, 43)

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