Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Biologic scaffolds from different tissue sources inhibit tumor formation but do not affect cancer cell viability.
  • Fig. S2. UBM implantation does not promote tumor growth in an orthotopic breast cancer resection model.
  • Fig. S3. CD4+ T cell purity in adoptive transfer experiments.
  • Fig. S4. Differentially expressed genes in T cells sorted from saline and UBM tumors.
  • Fig. S5. T lymphocyte characterization in the UBM-tumor microenvironment and DLNs.
  • Fig. S6. Gating strategy for myeloid cell analysis with flow cytometry.
  • Fig. S7. Detailed analysis of macrophage polarization.
  • Fig. S8. Macrophage depletion using clodronate liposomes.
  • Fig. S9. Differentially expressed genes in macrophages sorted from UBM and saline tumors.
  • Fig. S10. The synthetic adjuvant material microenvironment is distinct from UBM.
  • Fig. S11. Acellular UBM induces type 2 immune responses.
  • Fig. S12. B16-F10 cell titration with the combination of UBM and anti–PD-1.
  • Fig. S13. Tumor rejection occurs in the UBM microenvironment with anti–PD-1 treatment and leads to protection on rechallenge.
  • Fig. S14. UBM dose response for tumor growth inhibition.
  • Fig. S15. Gating strategy for T cell and macrophage cell sorting.
  • Table S3. Antibodies used in immunofluorescence histology.
  • Table S4. Antibodies used in flow cytometry experiments.

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Sorted T cell gene expression data.
  • Table S2 (Microsoft Excel format). Sorted macrophage gene expression data.
  • Table S5 (Microsoft Excel format). Primary data.