Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Demonstration of in vivo induction of the PfnrS using green fluorescent protein as a reporter in EcN strain.
  • Fig. S2. EcN strains lacking the thyA gene fail to grow in rich media lacking exogenous thymidine.
  • Fig. S3. Differential gene expression for SYNB1020 compared to EcN for genes in the l-arg biosynthesis pathway.
  • Fig. S4. Dose-dependent fecal excretion of EcN and SYNB1020 strains in mice.
  • Fig. S5. Study design of a randomized, double-blinded, placebo-controlled study to assess the safety, tolerability, and pharmacodynamics of SYNB1020 in healthy volunteers.
  • Fig. S6. Change from baseline in C-reactive protein.
  • Fig. S7. Time-matched change from baseline for diastolic blood pressure.
  • Fig. S8. Time-matched change from baseline for systolic blood pressure.
  • Legend for table S1
  • Table S2. Quantification of viable SYNB1020KR in mouse cecolonic samples over time.
  • Table S3. Average ECNStrR or SYNB1020KR CFU recovered from GI contents of mice dosed with each strain.
  • Table S4. Activity rate of SYNB1020KR recovered from mouse cecum and colon.
  • Table S5. Arginine pathway metabolites in mouse and nonhuman primates.
  • Table S6. Clinical and laboratory results from a 28-day oral toxicity study of SYNB1020 in mice.
  • Table S7. Mice with detectable SYNB1020 DNA in tissues as measured by qPCR.
  • Table S8. Baseline demographics for the phase 1 study population.
  • Table S9. ECG QT interval in the phase 1, multiple-day, study population.
  • Table S10. Laboratory results for urea cycle products in the multiple-day cohort phase 1 study population.
  • Table S11. 15N metabolites in the phase 1 study population.
  • Table S12. Primers used to characterize SYNB1020 genetic modifications.
  • Table S13. Fermentation medium (FM1).
  • References (8190)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Differential gene expression comparisons of SYNB1020 and EcNΔthyA to EcN in vitro.