Supplementary Materials

The PDF file includes:

  • Detailed Materials and Methods
  • Fig. S1. Sample timeline for MRI analysis of patient data.
  • Fig. S2. Relation between RANO criteria, first tumor growth velocity, and survival.
  • Fig. S3. Validation of sex differences in correlations between presurgical D and rho and survival.
  • Fig. S4. Geometric illustration of JIVE decomposition.
  • Fig. S5. Joint structure of male and female GBM samples relative to TCGA subtype signatures.
  • Fig. S6. Expression variation in TCGA data explained by the JIVE components.
  • Fig. S7. Identification of the sex-specific clusters by consensus clustering for female and male GBMs.
  • Fig. S8. Sex-specific cluster survival benefits in merged TCGA, GSE16011, and GSE13041 datasets.
  • Fig. S9. fc3-defining genes and survival.
  • Fig. S10. mc5-defining genes and survival.
  • Fig. S11. TMZ, etoposide, VCR, and CCNU dose-response curves for five male and four female primary GBM cell lines.
  • Fig. S12. Western blot analysis of MGMT expression in female and male patient-derived GBM cell lines.
  • Fig. S13. Distribution of the coefficients of variation in the TCGA data.
  • Fig. S14. Analysis flowchart.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Sex and TCGA subtype assignments in the four datasets.
  • Table S2 (Microsoft Excel format). Gene expression signatures for sex-specific clusters.
  • Table S3 (Microsoft Excel format). Median survival time, P values, and HRs associated with survival differences in sex-specific clusters.
  • Table S4 (Microsoft Excel format). Survival by IDH1 status—Female IDH1 WT.
  • Table S5 (Microsoft Excel format). Survival by IDH1 status—Male IDH1 WT.
  • Table S6 (Microsoft Excel format). Survival by IDH1 status—Male IDH1 mutant.
  • Table S7 (Microsoft Excel format). Survival by IDH1 status—Female IDH1 mutant.
  • Table S8 (Microsoft Excel format). Survival analysis of sex—IDH1 interaction.
  • Table S9 (Microsoft Excel format). Pathway analysis of fc3 and mc5.
  • Table S10 (Microsoft Excel format). Statistical analysis of the interaction between sex and chemotherapy treatment in vivo.
  • Table S11 (Microsoft Excel format). Patient-specific proliferation, invasion, hypoxia, necrosis, angiogenesis (PINHA) values.
  • Table S12 (Microsoft Excel format). Parameters for calculation of tumor growth velocities.