Supplementary Materials

The PDF file includes:

  • Fig. S1. Schematic demonstrating the HNW assay workflow.
  • Fig. S2. Determination of assay sensitivity for Ebola, Lassa, and malaria HNW assays using recombinant antigens in assay buffer.
  • Fig. S3. Ebola inclusivity testing using the triplex assay with high concentrations of Sudan virus and Bundibugyo virus and a negative control.
  • Fig. S4. Signals obtained from recombinant antigens spiked into matched human blood, plasma, or serum that was provided by a single donor.
  • Fig. S5. Signal intensities for malaria and Ebola antigens spiked into duplex and triplex assay formats using blood as a test medium.
  • Fig. S6. ROC curves for the HNW malaria assay.
  • Fig. S7. Example SERS spectra from a clinical sample assay that indicated a single infectious agent.
  • Fig. S8. Example SERS spectra from a clinical sample assay that indicated a co-infection with malaria and EBOV.
  • Table S1. Reference and passage information for viruses used in the NHP model.

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Other Supplementary Material for this manuscript includes the following:

  • Table S2 (Microsoft Excel format). Raw data for experiments where N < 20.