Supplementary Materials

The PDF file includes:

  • Fig. S1. The surface mesothelium remains after gentle abrasion injury.
  • Fig. S2. The surface mesothelium remains on buttons after placement.
  • Fig. S3. PDPN and MSLN are exclusive to surface mesothelium.
  • Fig. S4. The surface mesothelium buttons are MSLN+ after placement.
  • Fig. S5. The surface mesothelium thickens in response to injury.
  • Fig. S6. The surface mesothelium thickens in response to injury.
  • Fig. S7. Chimerism in parabiotic mice.
  • Fig. S8. Circulating cells do not contribute significantly to adhesions.
  • Fig. S9. EdU+ cells are found in areas of tissue thickening.
  • Fig. S10. Scanning electron microscopy of peritoneal buttons.
  • Fig. S11. Scanning electron microscopy of peritoneal buttons.
  • Fig. S12. Large block-face scanning electron microscopy of peritoneal buttons.
  • Fig. S13. CFSE treatment labels only surface mesothelial cells.
  • Fig. S14. CFSE-labeled mesothelial cells contribute to adhesions.
  • Fig. S15. PDPN+ ActinCreER; R26VT2/GK3 cells give rise to adhesions.
  • Fig. S16. In vitro mesothelial transition.
  • Fig. S17. Anti-MSLN antibody specifically binds the surface mesothelium.
  • Fig. S18. Antibodies against host IgG do not bind mesothelial cells.
  • Fig. S19. Adhesion scoring characterized by surface area contact and molecular markers.
  • Fig. S20. K19+PDPN+ mesothelium persists after antibody treatment.
  • Fig. S21. Mesothelium persists after antibody treatment.
  • Fig. S22. ECM genes are down-regulated after adhesion induction.
  • Fig. S23. Validation of enriched genes via immunofluorescence.
  • Fig. S24. Transcript levels of HIF1A stabilization proteins.
  • Fig. S25. Mesothelial in vitro culture assay.
  • Fig. S26. HIF1A and mesothelial gene targets after HIF1α inhibition.
  • Fig. S27. HIF1A and mesothelial gene targets after HIF1α inhibition.
  • Fig. S28. Echinomycin does not affect DNA replication.
  • Fig. S29. A proposed mesothelial centric model of adhesion formation.
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Date file S1 (Microsoft Excel format). Source data.
  • Movie S1 (.avi format). 3D rendering of light sheet microscopy imaging of injured surface mesothelium.
  • Movie S2 (.avi format). 3D rendering of light sheet microscopy imaging of injured surface mesothelium.