Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Optimization of alginate coating composition.
  • Fig. S2. Analysis of fluids recovered from microneedles accurately reflects surrounding solution concentrations.
  • Fig. S3. TRM characterization in the blood and skin compartments in OVA-immunized mice.
  • Fig. S4. Cell recruitment is enhanced with the inclusion of adjuvants in the alginate hydrogel coating.
  • Fig. S5. ICMV characterization and increased recruitment of cells into sampling microneedles when ICMVs encapsulating antigen and adjuvant are embedded in the alginate layer of sampling microneedles.
  • Fig. S6. The activity of polyI:C is retained upon incorporation within sampling microneedles.
  • Fig. S7. SSMNs containing adjuvants activate recruited APCs.
  • Fig. S8. SSMN application for up to 48 hours does not change the immune status of the animal.
  • Fig. S9. Gating strategy for cells obtained from sampling microneedles applied to human skin.
  • Reference (39)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Primary data.