Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Monocytes, neutrophils, eosinophils, and T cells in recolonized mice.
  • Fig. S2. Characterization of DCs and distribution of monocytes and macrophages in Ccr2−/− mice.
  • Fig. S3. Gene expression profiles of mature colonic macrophages from antibiotic-treated and GF mice.
  • Fig. S4. Sustained immune dysfunction in recolonized mice repolarizes immune responses to infection.
  • Fig. S5. Recolonization of antibiotic-treated mice causes long-term disruption of the intestinal microbiota.
  • Fig. S6. The SCFA butyrate alleviates antibiotic-associated immune dysfunction.
  • Fig. S7. TH1 polarization in il-10−/− recolonized mice.
  • Table S1. Antibodies.
  • Table S2. qRT-PCR primer sequences for macrophage gene expression.
  • Table S3. Primer sequences for 16S bacterial sequencing.
  • References (4860)

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Other Supplementary Material for this manuscript includes the following:

  • Table S4 (Microsoft Excel format). Primary data.
  • Data file S1 (Microsoft Excel format). Bacterial sequencing reads.