Supplementary Materials

The PDF file includes:

  • Methods
  • Fig. S1. Progressive lengthening of the linker leads to loss of Gram selectivity.
  • Fig. S2. NBD-PMX has improved signal-to-noise ratio at lower concentrations in the presence of pulmonary surfactant.
  • Fig. S3. NBD-PMX binding with PMX-resistant strains of B. cenocepacia is reduced compared to P. aeruginosa.
  • Fig. S4. NBD-PMX demonstrates no cell membrane toxicity and no in vivo toxicity after intratracheal instillation in mice.
  • Fig. S5. OEM coupled with NBD-PMX enables bioburden estimation in the distal ovine lung.
  • Fig. S6. NBD-PMX labels Gram-negative bacteria endobronchially.
  • Fig. S7. Ex vivo confocal labeling of species from BAL in patient D11.
  • Fig. S8. Synthetic route of NBD-PMX probe.
  • Fig. S9. Analytical high-performance liquid chromatography of NBD-PMX.
  • Fig. S10. Analytical high-resolution mass spectrometry of NBD-PMX.
  • Table S1. NBD-PMX demonstrates no changes in weight, hematological, coagulation, or clinical chemistry parameters in GLP toxicity study.
  • Table S2. NBD-PMX is stable in aqueous formulation over 24 months.
  • Table S3. Bronchiectasis patient demographics, blood results, adverse events, and microbiological growth.
  • Table S4. ICU patient demographics for six patients included in the analysis, blood results, adverse events, and microbiological growth.
  • Legends of data files S1 and S2
  • Legends of movies S1 to S13
  • Reference (54)

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (.zip format). MATLAB file containing image analysis algorithm for preclinical images.
  • Data file S2 (.zip format). MATLAB file containing image analysis algorithm for clinical images.
  • Movie S1 (.avi format). GFP-expressing S. aureus instilled in ovine bronchopulmonary segment and imaged with OEM.
  • Movie S2 (.avi format). Patient D1 imaged after administration of NBD-PMX (no bacterial signal seen).
  • Movie S3 (.avi format). Patient D2 imaged after administration of NBD-PMX (bacterial signal seen).
  • Movie S4 (.avi format). Patient D3 imaged after administration of NBD-PMX (no bacterial signal seen).
  • Movie S5 (.avi format). Patient D4 imaged after administration of NBD-PMX (no bacterial signal seen).
  • Movie S6 (.avi format). Patient D5 imaged after administration of NBD-PMX (bacterial signal seen).
  • Movie S7 (.avi format). Patient D6 imaged after administration of NBD-PMX (bacterial signal seen).
  • Movie S8 (.avi format). ICU patient D7 imaged after administration of NBD-PMX (bacterial signal seen).
  • Movie S9 (.avi format). ICU patient D8 imaged after administration of NBD-PMX (no bacterial signal seen).
  • Movie S10 (.avi format). ICU patient D9 imaged after administration of NBD-PMX (no bacterial signal seen).
  • Movie S11 (.avi format). ICU patient D11 imaged after administration of NBD-PMX (bacterial signal seen).
  • Movie S12 (.avi format). ICU patient D12 imaged after administration of NBD-PMX (no bacterial signal seen).
  • Movie S13 (.avi format). ICU patient D13 imaged after administration of NBD-PMX (no bacterial signal seen).