Supplementary Materials
The PDF file includes:
- Materials and Methods
- Fig. S1. Itgbl1 expression pattern in facial cartilage tissues and homology of ITGBL1 across vertebrates.
- Fig. S2. Neural crest cell migration, hypertrophic chondrocyte development, and osteogenic differentiation after ITGBL1 depletion.
- Fig. S3. Fibronectin deposition after ITGBL1 depletion.
- Fig. S4. Itgbl1 expression during chondrogenesis analyzed after siRNA transfection.
- Fig. S5. ITGBL1 promotes chondrogenesis.
- Fig. S6. Gene expression analysis during chondrogenesis of Itgbl1-overexpressing hBMSCs.
- Fig. S7. ITGBL1 reduces cell-ECM adhesion.
- Fig. S8. ITGBL1-induced integrin inactivation is not recovered by integrin-activating antibody.
- Fig. S9. ITGBL1 inhibits focal adhesion complex formation.
- Fig. S10. Relative expression of integrin subunits in human primary chondrocytes and efficacy of siRNAs used in Fig. 5 (F and G).
- Fig. S11. Itgbl1 expression pattern in embryonic mouse limbs and subcellular localization in ATDC5 cells.
- Fig. S12. Cell viability and efficiency of adenovirus delivery in Ad-Itgbl1–infected chondrocytes and cartilage in mice.
- Fig. S13. ITGBL1 expression protects cartilage tissue against OA development in mice.
- Fig. S14. Scoring of synovial inflammation in the OA mouse model.
- Fig. S15. Human OA cartilage information.
- Table S1. List of the top 100 putative secreted proteins identified by transcriptome analysis.
- References (74, 75)
Other Supplementary Material for this manuscript includes the following:
- Table S2 (Microsoft Excel format). Individual subject-level data presented in the figures.
