Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Itgbl1 expression pattern in facial cartilage tissues and homology of ITGBL1 across vertebrates.
  • Fig. S2. Neural crest cell migration, hypertrophic chondrocyte development, and osteogenic differentiation after ITGBL1 depletion.
  • Fig. S3. Fibronectin deposition after ITGBL1 depletion.
  • Fig. S4. Itgbl1 expression during chondrogenesis analyzed after siRNA transfection.
  • Fig. S5. ITGBL1 promotes chondrogenesis.
  • Fig. S6. Gene expression analysis during chondrogenesis of Itgbl1-overexpressing hBMSCs.
  • Fig. S7. ITGBL1 reduces cell-ECM adhesion.
  • Fig. S8. ITGBL1-induced integrin inactivation is not recovered by integrin-activating antibody.
  • Fig. S9. ITGBL1 inhibits focal adhesion complex formation.
  • Fig. S10. Relative expression of integrin subunits in human primary chondrocytes and efficacy of siRNAs used in Fig. 5 (F and G).
  • Fig. S11. Itgbl1 expression pattern in embryonic mouse limbs and subcellular localization in ATDC5 cells.
  • Fig. S12. Cell viability and efficiency of adenovirus delivery in Ad-Itgbl1–infected chondrocytes and cartilage in mice.
  • Fig. S13. ITGBL1 expression protects cartilage tissue against OA development in mice.
  • Fig. S14. Scoring of synovial inflammation in the OA mouse model.
  • Fig. S15. Human OA cartilage information.
  • Table S1. List of the top 100 putative secreted proteins identified by transcriptome analysis.
  • References (74, 75)

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Other Supplementary Material for this manuscript includes the following:

  • Table S2 (Microsoft Excel format). Individual subject-level data presented in the figures.