Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Two-dimensional photoluminescence plots of isolated SWCNT chiralities.
  • Fig. S2. Identification of ssCTTC3TTC-(9,4) as a potential probe for endolysosomal lipid accumulation.
  • Fig. S3. Representative emission spectra of ssCTTC3TTC-(9,4) at a neutral and acidic pH.
  • Fig. S4. ssCTTC3TTC-(9,4) photoluminescent response in solution.
  • Fig. S5. Atomic force microscopy of ssCTTC3TTC-(9,4) complexes.
  • Fig. S6. Initial REMD configurations.
  • Fig. S7. Analysis of REMD simulations.
  • Fig. S8. Mean emission wavelength of ssCTTC3TTC-(9,4) in live RAW 264.7 macrophages treated with inhibitors.
  • Fig. S9. Effect of inhibitors on ssCTTC3TTC-(9,4) emission spectra in solution.
  • Fig. S10. Reporter response to HPβCD treatment in MEFs.
  • Fig. S11. Assessing the effect of ssCTTC3TTC-(9,4) on lipoprotein hydrolysis.
  • Fig. S12. NIR in vivo spectroscope.
  • Fig. S13. Representative images of liver tissue from wild-type and ASMKO mice.
  • Fig. S14. Representative images of hepatic tissues from wild-type and ASMKO mice stained with the hepatocyte marker CK8/18.
  • Fig. S15. Representative images of hepatic tissue from wild-type and ASMKO mice stained with the KC marker F4/80.
  • Fig. S16. Representative images from H&E-stained tissues from the livers of wild-type and Npctm(I1061T)Dso mice.
  • Fig. S17. Representative images of hepatic tissue from wild-type and Npctm(I1061T)Dso mice stained with the hepatocyte marker CK8/18.
  • Fig. S18. Representative images of hepatic tissues from wild-type and Npctm(I1061T)Dso mice stained with the KC marker F4/80.
  • Fig. S19. Exponential fits of the decrease in ssCTTC3TTC-(9,4) emission wavelength after intravenous injection of 200 μg of oxLDL in mice.
  • Fig. S20. Testing the effects of elevated lipid concentrations on reporter emission in whole blood.
  • Fig. S21. Weights of male C57BL/6 mice fed SC or WD with HFCS.
  • Fig. S22. Representative images of hepatic tissues stained with CK8/18 from mice fed SC or WD with HFCS for 1 month.
  • Fig. S23. Representative images of hepatic tissues stained with F4/80 from mice fed SC or WD with HFCS for 1 month.
  • Fig. S24. Representative images of hepatic tissues stained with CK8/18 from mice fed SC or WD with HFCS for 3 months.
  • Fig. S25. Representative images of hepatic tissues stained with F4/80 from mice fed SC or WD with HFCS for 3 months.
  • Fig. S26. Representative images of H&E-stained liver tissues from C57BL/6 mice fed SC or WD with HFCS for 1 month.
  • Fig. S27. Representative H&E-stained liver tissues from mice fed SC or WD with HFCS for 3 months.
  • Fig. S28. In vivo emission spectra of the reporter from the liver of mice fed SC or WD with HFCS for 1 to 3 months.
  • Fig. S29. Analysis of intercellular heterogeneity of KC lipid content in vivo.
  • Fig. S30. Reporter presence in relation to lipogranulomas.
  • Fig. S31. Weight and serum chemistry measurements of mice fed SC or WD with HFCS for 3 months.
  • Fig. S32. Serum chemistry measurements and weights of reporter- and vehicle-injected mice in a model of hepatic injury.
  • Fig. S33. Relative hepatic expression of genes involved in the innate immune response after injection with ssCTTC3TTC-(9,4) or PBS.
  • Fig. S34. Representative images of H&E-stained liver tissue from C57BL/6 mice fed SC or WD with HFCS for 2 weeks.
  • Fig. S35. Representative images of liver tissue stained with Oil Red O neutral lipid stain.
  • Fig. S36. Representative images of hepatic tissue from mice fed SC or WD with HFCS for 2 weeks stained with the hepatocyte marker CK8/18.
  • Fig. S37. Representative images of hepatic tissue from mice fed SC or WD with HFCS for 2 weeks stained with the KC marker F4/80.
  • Fig. S38. Representative H&E sections of livers from mice shown in Fig. 5E.
  • Table S1. Average reporter emission center wavelengths measured from wild-type and ASMKO mice in vivo, ex vivo¸ and in frozen sections of resected liver tissue.
  • Table S2. Average reporter emission center wavelengths measured from wild-type and Npctm(I1061T)Dso mice in vivo, ex vivo, and in frozen sections of resected liver tissue.
  • References (4756)

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S3 (Microsoft Excel format). Individual mouse data.