Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. HDM-treated blt1−/− mice show ameliorated AHR and airway inflammation relative to wild-type controls.
  • Fig. S2. HDM-treated lta4h−/− mice have comparable lung tissue inflammation to littermate controls despite exhibiting an exacerbated AHR.
  • Fig. S3. HDM-treated lta4h−/− mice exhibit reduced concentrations of TH2 inflammatory mediators in their lung tissue relative to their littermate controls.
  • Fig. S4. Lipoxin A4 and cysteinyl leukotrienes are not elevated in HDM-treated lta4h−/− mice.
  • Fig. S5. Comparable neutrophilic inflammation and mediators in HDM-exposed wild-type and lta4h−/− mice.
  • Fig. S6. HDM-treated lta4h−/− mice exhibit augmented airway epithelial remodeling relative to littermate controls.
  • Fig. S7. Comparable smooth muscle and collagen deposition in HDM-treated wild-type and lta4h−/− mice.
  • Fig. S8. HDM-treated blt1−/− mice do not show PGP accumulation or increased epithelial height compared to wild-type mice.
  • Fig. S9. Direct application of AcPGP to bronchial epithelial cells increases cell proliferation and confluence.
  • Fig. S10. A protease imbalance could facilitate PGP accumulation in severe asthmatics.
  • Fig. S11. Flow cytometry gating strategy used to define myeloid cell populations.
  • Fig. S12. Flow cytometry gating strategy used to define CD4+ T cell population and associated markers.
  • Fig. S13. Flow cytometry gating strategy used to define ILC2 population and associated markers.
  • Table S1. Clinical features of patients with severe asthma and healthy subjects from the primary cohort (UK) included in the study.
  • Table S2. Clinical features of patients with severe and nonsevere asthma from the secondary cohort (United States) included in the study.
  • Table S3. Differential cell types were defined by specific cell surface markers using flow cytometry.
  • Legend for movie S1

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Other Supplementary Material for this manuscript includes the following:

  • Table S4 (Microsoft Excel format). Primary data.
  • Movie S1 (.avi format). Real-time imaging of AcPGP-induced changes in epithelial cell confluence and morphology.