Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Synthesis of FI-DNB1, FI-DNB2, and FI-MNB.
  • Fig. S2. Synthesis of Rd-DNB and Rd-MNB.
  • Fig. S3. Synthesis of TG-DNB1.
  • Fig. S4. Synthesis of TG-DNB2.
  • Fig. S5. Synthesis of CDG-DNB1.
  • Fig. S6. Synthesis of CDG-DNB2.
  • Fig. S7. Synthesis of CDG-DNB3.
  • Fig. S8. SDS-PAGE analysis of purified M. smegmatis DprE1.
  • Fig. S9. DprE1 DNA sequence alignment.
  • Fig. S10. Microscopic imaging and flow cytometry analysis of bacteria labeled by TG and TG-DNB analogs.
  • Fig. S11. Characterization of TG-DNB2 in M. smegmatis.
  • Fig. S12. Growth curve of M. smegmatis and BCG.
  • Fig. S13. Inhibition study of TG-DNB2 with DNB1.
  • Fig. S14. Characterization of CDG-1 and CDG-DNB analogs in bacteria.
  • Fig. S15. Western blots detecting the expression of TEM-1 Bla and BlaC in transformed E. coli.
  • Fig. S16. Labeling of BCG and H37Rv by CDG-DNB2.
  • Fig. S17. Inhibition study with BlaC KO, BlaC compensated, and control vector transformed H37Rv and DNB1.
  • Fig. S18. Labeling of Mtb in processed sputum by CDG-DNB2.
  • Fig. S19. Evaluation of selectivity of CDG-DNB2/3 for BlaC.
  • Fig. S20. Fluorescence intensity of CDG-DNB3 and TG-DNB2 with or without clavulanic acid, DNB1, or DprE1.
  • Fig. S21. Characterization of CDG-DNB3 in M. smegmatis, BCG, and TEM-1 Bla or BlaC transformed E. coli.
  • Fig. S22. Specific labeling of viable BCG by CDG-DNB3.
  • Fig. S23. Labeling of Mtb in processed sputum by CDG-DNB3.
  • Fig. S24. Overall layout and the functional regions of the microfluidic bacteria-counting device.
  • Table S1. List of NTMs stained with CDG-DNB3.
  • Legend for Movies S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Table S2 (Microsoft Excel format). List of individual subject-level data shown on bar graphs in all figures.
  • Movie S1 (.avi format). Real-time fluorescence imaging of CDG-DNB2–treated BCG aggregates.
  • Movie S2 (.avi format). Real-time bright-field imaging of CDG-DNB2–treated BCG aggregates in movie S1.
  • Movie S3 (.avi format). Infection of macrophages by CDG-DNB3–labeled individual BCG bacilli.
  • Movie S4 (.mp4 format). Automated counting of CDG-DNB3–labeled BCG bacilli with microfluidic chip.
  • Appendix S1 (PDF format). 1H and 13C nuclear magnetic resonance and mass spectroscopy spectra.