Supplementary Materials

The PDF file includes:

  • Fig. S1. Tolerance of E. faecium to ethanol exposure.
  • Fig. S2. Isopropanol tolerance phenotype summary for 129 genome-sequenced E. faecium isolates.
  • Fig. S3. Core and pan-genome analysis of 129 E. faecium genomes.
  • Fig. S4. Growth curves of mutants.
  • Legends for tables S1 to S3
  • Table S4. Oligonucleotides used in this study.
  • Table S5. Bacterial strains and plasmids used for E. faecium mutagenesis.
  • Table S6. Data for E. faecium CD50 experiments.
  • Table S7. Data for E. faecium density (CFU/cm2) on the cage floor pre- and posttreatments.
  • Table S8. Data for mice colonized by E. faecium after either water or alcohol cage floor disinfection.
  • Table S9. Data for E. faecium (CFU/ml) remaining over time after exposure to isopropanol.
  • Table S10. Data for change in doubling times of E. faecium mutants grown in the presence of isopropanol.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Summary of E. faecium strains used in this study, with associated isopropanol tolerance data.
  • Table S2 (Microsoft Excel format). Pairwise comparisons of high-low alcohol-tolerant E. faecium.
  • Table S3 (Microsoft Excel format). DAPC analysis based on ortholog comparisons versus alcohol tolerance.

[Download Tables S1 to S3]