Supplementary Materials

The PDF file includes:

  • Fig. S1. Synthesis of botulinum peptide conjugates using a stapling bridge.
  • Fig. S2. SP-BOT has no effect on mechanical threshold in the contralateral paw.
  • Fig. S3. Effect of different doses of intrathecal SP-BOT or Derm-BOT on CFA-induced hypersensitivity.
  • Fig. S4. Intrathecal injection of unconjugated BOT LcTd (Neg-BITOX) without a receptor binding domain had no effect on inflammatory hyperalgesia.
  • Fig. S5. cSNAP25-positive neurons after SP-BOT or Derm-BOT intrathecal injection.
  • Fig. S6. SP-BOT or Derm-BOT intrathecal injection does not induce glial activation in the dorsal horn.
  • Fig. S7. Quantification of MOR fluorescence intensity.
  • Fig. S8. Effect of SP-BOT injection on withdrawal threshold plotted as force.
  • Fig. S9. Effect of Derm-BOT injection on withdrawal threshold plotted as force.
  • Table S1. Statistical analysis for Figs. 1, 2, 3, and 5 and figs. S2 and S7.
  • Table S2. Maximum possible effect.
  • Legend for Table S3

[Download PDF]

Other Supplementary Material for this manuscript includes the following: