Supplementary Materials

Supplementary Material for:

Preclinical assessment of antiviral combination therapy in a genetically humanized mouse model for hepatitis delta virus infection

Benjamin Y. Winer, Elham Shirvani-Dastgerdi, Yaron Bram, Julie Sellau, Benjamin E. Low, Heath Johnson, Tiffany Huang, Gabriela Hrebikova, Brigitte Heller, Yael Sharon, Katja Giersch, Sherif Gerges, Kathleen Seneca, Mihai-Alexandru Pais, Angela S. Frankel, Luis Chiriboga, John Cullen, Ronald G. Nahass, Marc Lutgehetmann, Jared E. Toettcher, Michael V. Wiles, Robert E. Schwartz, Alexander Ploss*

*Corresponding author. Email: aploss{at}

Published 27 June 2018, Sci. Transl. Med. 10, eaap9328 (2018)
DOI: 10.1126/scitranslmed.aap9328

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Schematic of the modified BAC containing the SLC10A1 gene used for generating the hNTCP tg mice on the NRG and C57BL/6 backgrounds.
  • Fig. S2. hNTCP expression in liver and other organs of hNTCP-BAC mice on the C57BL/6 and NRG backgrounds.
  • Fig. S3. EdC labeling of the HBV genome does not affect viral fitness.
  • Fig. S4. Quantification of HBV replication intermediates in HBV-infected hNTCP-NRG mice.
  • Fig. S5. Delivery of 1.3× HBV plasmid leads to sustained HBsAg secretion over time.
  • Fig. S6. HBsAg levels remain stable in HDV-infected hNTCP/BAC/1.3× HBV HDD mice over the experimental time course.
  • Fig. S7. Evidence for RNA editing in HDV genomes isolated from livers of HDV-infected hNTCP/BAC/1.3× HBV-NRG mice.
  • Fig. S8. Adenoviral delivery of HBV envelope proteins leads to expression of all three forms of HBsAg large (L), medium (M), and small (S).
  • Fig. S9. Assessment of HDVAg in HDV-challenged hNTCP/BAC-NRG mice.
  • Fig. S10. Longitudinal sequence analysis of hypervariable domain in hNTCP/BAC/1.3× HBV HDD NRG mice.
  • Fig. S11. Longitudinal sequence analysis of Rbz domain in hNTCP/BAC/1.3× HBV HDD NRG mice.
  • Fig. S12. Predicted effect of the A/G mutation at position 46 of HDV Rbz on RNA secondary structure.
  • Fig. S13. Longitudinal sequence analysis of HDVAg domain in hNTCP/BAC/1.3× HBV HDD NRG mice.
  • Fig. S14. Longitudinal sequence analysis of HDVAg domain in immunocompetent hNTCP/BAC/1.3× HBV C57BL/6 mice.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. (Microsoft Excel format). Primary data.