Supplementary Materials

Supplementary Material for:

Probiotic strains detect and suppress cholera in mice

Ning Mao, Andres Cubillos-Ruiz, D. Ewen Cameron, James J. Collins*

*Corresponding author. Email: jimjc{at}

Published 13 June 2018, Sci. Transl. Med. 10, eaao2586 (2018)
DOI: 10.1126/scitranslmed.aao2586

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Homolactic fermentation of glucose by L. lactis leads to growth inhibition of V. cholerae.
  • Fig. S2. L. lactisV. cholerae coculture system.
  • Fig. S3. Lactic acid displays a bactericidal effect on V. cholerae.
  • Fig. S4. L. lactis transit dynamics in mouse gut.
  • Fig. S5. Litter variation in infant mouse survival study.
  • Fig. S6. Growth and acidification capability of L. lactis strains used in this study.
  • Fig. S7. V. cholerae colonization in infant mice treated with lactic acid.
  • Fig. S8. Survival of V. cholerae–infected infant mice under acid-neutralizing feeding regimens.
  • Fig. S9. HR design and screening.
  • Fig. S10. xyltet promoter testing in L. lactis and circuit-tuning with different TetR translation rates.
  • Fig. S11. Specificity of signal activation of the HR4M HR to other Vibrio spp. and non–Vibrio spp.
  • Fig. S12. Metabolic burden of L. lactis harboring engineered circuits.
  • Fig. S13. Growth rate of L. lactis strains used in this study.
  • References (3651)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Primary data.