Supplementary Materials

Supplementary Material for:

Anchor peptide captures, targets, and loads exosomes of diverse origins for diagnostics and therapy

Xianjun Gao, Ning Ran, Xue Dong, Bingfeng Zuo, Rong Yang, Qibing Zhou, Hong M. Moulton, Yiqi Seow, HaiFang Yin*

*Corresponding author. Email: haifangyin{at}tmu.edu.cn

Published 6 June 2018, Sci. Transl. Med. 10, eaat0195 (2018)
DOI: 10.1126/scitranslmed.aat0195

This PDF file includes:

  • Fig. S1. In vitro biopanning for exosomal anchor peptides.
  • Fig. S2. Characterization of myotube-derived exosomes and coimmunoprecipitation assay for verifying the specific binding of CP05 to CD63.
  • Fig. S3. Validation of flow cytometry assay for exosome measurement and quantitative analysis of CD63 protein on exosomal surface.
  • Fig. S4. Characterization of M12-CP05–modified exosomes (EXOM12) and exosomes derived from Hepa1-6, human cells, and serum.
  • Fig S5. Characterization of CP05-PMO–modified exosomes (EXOPMO) and colocalization of DiR-labeled exosomes with dystrophin-positive fibers.
  • Fig S6. Comparison between single and triple intravenous injection of EXOPMO at the dose of 12.5 mg PMO/kg per week in mdx mice.
  • Table S1. Nomenclature and sequences of peptides and PMO used in the study.

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