Supplementary Materials

Supplementary Material for:

The antiprotease SPINK7 serves as an inhibitory checkpoint for esophageal epithelial inflammatory responses

Nurit P. Azouz, Mario A. Ynga-Durand, Julie M. Caldwell, Ayushi Jain, Mark Rochman, Demetria M. Fischesser, Leanne M. Ray, Mary C. Bedard, Melissa K. Mingler, Carmy Forney, Matthew Eilerman, Jonathan T. Kuhl, Hua He, Jocelyn M. Biagini Myers, Vincent A. Mukkada, Philip E. Putnam, Gurjit K. Khurana Hershey, Leah C. Kottyan, Ting Wen, Lisa J. Martin, Marc E. Rothenberg*

*Corresponding author. Email: rothenberg{at}cchmc.org

Published 6 June 2018, Sci. Transl. Med. 10, eaap9736 (2018)
DOI: 10.1126/scitranslmed.aap9736

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Differential sequence and expression of SPINK5 and SPINK7.
  • Fig. S2. SPINK5 and SPINK7 are esophageal epithelial differentiation markers.
  • Fig. S3. Analysis of the SPINK7 transcriptome reveals a differentiation defect.
  • Fig. S4. Loss of SPINK7 induces intercellular dilated spaces and barrier impairment.
  • Fig. S5. Analysis of the SPINK7 transcriptome reveals overlaps with the EoE and IL-13–induced transcriptomes.
  • Fig. S6. SPINK7 gene silencing induces cytokine release.
  • Fig. S7. Generation of SPINK7 KO EPC2 cells.
  • Fig. S8. uPA administration does not alter barrier integrity or promote overexpression of EMT markers in the ALI culture system.
  • Fig. S9. uPAR expression on immune cells and the effect of uPA on eosinophils.
  • Fig. S10. A summary of the effect of barrier gene deficiency on SPINK7 expression and a model of SPINK7 pathway in EoE.
  • Legends for movies S1 and S2
  • Legends for tables S1 to S4
  • References (4046)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Immunofluorescence of junctional proteins of differentiated control EPC2 cells.
  • Movie S2 (.avi format). Immunofluorescence of junctional proteins of differentiated SPINK7-silenced EPC2 cells.
  • Table S1 (Microsoft Excel format). Correlation between the expression of SPINK5 or SPINK7 with EoE signature genes.
  • Table S2 (Microsoft Excel format). SPINK7 transcriptome.
  • Table S3 (Microsoft Excel format). Differentiation transcriptome.
  • Table S4 (Microsoft Excel format). CRISPR/Cas9 SPINK7 KO transcriptome.

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