Supplementary Materials

Supplementary Material for:

A common antimicrobial additive increases colonic inflammation and colitis-associated colon tumorigenesis in mice

Haixia Yang, Weicang Wang, Kymberleigh A. Romano, Min Gu, Katherine Z. Sanidad, Daeyoung Kim, Jun Yang, Birgitta Schmidt, Dipak Panigrahy, Ruisong Pei, Derek A. Martin, E. Ilker Ozay, Yuxin Wang, Mingyue Song, Bradley W. Bolling, Hang Xiao, Lisa M. Minter, Guang-Yu Yang, Zhenhua Liu, Federico E. Rey,* Guodong Zhang*

*Corresponding author. Email: guodongzhang{at}umass.edu (G.Z.); ferey{at}wisc.edu (F.E.R.)

Published 30 May 2018, Sci. Transl. Med. 10, eaan4116 (2018)
DOI: 10.1126/scitranslmed.aan4116

This PDF file includes:

  • Materials and Methods
  • Fig. S1. LC-MS/MS analysis of TCS and its metabolite in mouse plasma.
  • Fig. S2. Effects of TCS on cytokines in plasma.
  • Fig. S3. Gating strategies used for analysis of major immune cell populations.
  • Fig. S4. Effects of TCS on DSS-induced colitis in C57BL/6 mice.
  • Fig. S5. Effects of TCS on colitis in Il-10−/− mice.
  • Fig. S6. Effects of TCS (80 ppm in diet) on AOM/DSS-induced colon tumorigenesis in mice.
  • Fig. S7. Effects of TCS (10 ppm in diet) on expression of PCNA and β-catenin in colon tumors.
  • Fig. S8. Effects of TCS on fecal microbiota in mice.
  • Fig. S9. Effects of TCS on B. infantis 272 growth.
  • Table S1. Composition of diet used in the animal experiments.
  • Table S2. Effects of TCS on composition of mouse microbiota at phylum levels.
  • Table S3. Effects of TCS on composition of mouse microbiota at genus levels.
  • Table S4. Sequences of primers used in qRT-PCR and 16S rRNA sequencing.

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S5 (Microsoft Excel format). Primary data.