Supplementary Materials

Supplementary Material for:

Derepression of co-silenced tumor suppressor genes by nanoparticle-loaded circular ssDNA reduces tumor malignancy

Jing Meng, Shuang Chen, Jing-xia Han, Qiang Tan, Xiao-rui Wang, Hong-zhi Wang, Wei-long Zhong, Yuan Qin, Kai-liang Qiao, Chao Zhang, Wan-feng Gao, Yue-yang Lei, Hui-juan Liu, Yan-rong Liu, Hong-gang Zhou, Tao Sun,* Cheng Yang*

*Corresponding author. Email: cheng.yang{at}nankai.edu.cn (C.Y.); tao.sun{at}nankai.edu.cn (T.S.)

Published 23 May 2018, Sci. Transl. Med. 10, eaao6321 (2018)
DOI: 10.1126/scitranslmed.aao6321

This PDF file includes:

  • Fig. S1. Kaplan-Meier survival analysis of KLF17, CDH1, and LASS2 expression in breast, lung, and ovarian cancer.
  • Fig. S2. Correlations between KLF17, CDH1, and LASS2 expression in breast cancer.
  • Fig. S3. The inhibitory effects of carcinogenic miRNAs on KLF17, CDH1, and LASS2 expression and cell proliferation in HeLa cells.
  • Fig. S4. miR-9 expression in different tumors and normal tissues.
  • Fig. S5. miR-9–targeted KLF17, CDH1, and LASS2.
  • Fig. S6. The relative expression of miR-9 in tumor cell lines.
  • Fig. S7. mRNA expression of KLF17, CDH1, and LASS2 in different cancer cell lines.
  • Fig. S8. The absorbance of HEK293, LO2, HUVEC, and HaCaT cells after CSSD-9 treatment.
  • Fig. S9. The inhibitory effect of CSSD-190, CSSD-21, CSSD-17, and CSSD-10 on HeLa cells.
  • Fig. S10. mRNA expression relative to control of chemokines/receptors and growth factors in tumor tissues treated with CSSD-9.
  • Fig. S11. White blood cell counts, liver function, and kidney function of mice under CSSD-9 treatment.
  • Fig. S12. The inhibitory effect of CSSD-9 on lung metastasis in an experimental model.
  • Fig. S13. Schematic diagram of the mechanisms underlying the antitumor activity of CSSD.
  • Table S1. The sequences of CSSD-9 and circR-9.
  • Table S2. Primers used in cloning (vector: pcDNA3.1).
  • Table S3. Primers for real time RT-PCR.

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