Supplementary Materials

Supplementary Material for:

The protective role of macrophage migration inhibitory factor in acute kidney injury after cardiac surgery

Christian Stoppe,* Luisa Averdunk, Andreas Goetzenich, Josefin Soppert, Arnaud Marlier, Sandra Kraemer, Jil Vieten, Mark Coburn, Ana Kowark, Bong-Song Kim, Gernot Marx, Steffen Rex, Akinobu Ochi, Lin Leng, Gilbert Moeckel, Andreas Linkermann, Omar El Bounkari, Alexander Zarbock, Jürgen Bernhagen,* Sonja Djudjaj, Richard Bucala, Peter Boor*

*Corresponding author. Email: christian.stoppe{at} (C.S.); juergen.bernhagen{at} (J.B.); pboor{at} (P.B.)

Published 16 May 2018, Sci. Transl. Med. 10, eaan4886 (2018)
DOI: 10.1126/scitranslmed.aan4886

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Flowchart.
  • Fig. S2. MIF and NGAL concentrations in cardiac surgery patients.
  • Fig. S3. Protective effects of MIF in an experimental model of AKI induced by bilateral ischemia and 24 hours of reperfusion.
  • Fig. S4. Up-regulated MLKL expression in Mif−/− mice.
  • Fig. S5. Cytotoxicity of 18 hours of hypoxia followed by 6 hours of normoxia in pmTECs.
  • Fig. S6. MIF receptors and phosphorylation of MLKL in tubular epithelial cells.
  • Fig. S7. Scheme of the proposed action of MIF in the setting of cardiac surgery–related AKI.
  • Table S1. Baseline characteristics of patients undergoing cardiac surgery.
  • Table S2. List of primary and secondary antibodies for Western blot, immunohistochemistry, and immunofluorescence staining.
  • References (5357)

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