Supplementary Materials

Supplementary Material for:

CD32 is expressed on cells with transcriptionally active HIV but does not enrich for HIV DNA in resting T cells

Mohamed Abdel-Mohsen, Leticia Kuri-Cervantes, Judith Grau-Exposito, Adam M. Spivak, Racheal A. Nell, Costin Tomescu, Surya Kumari Vadrevu, Leila B. Giron, Carla Serra-Peinado, Meritxell Genescà, Josep Castellví, Guoxin Wu, Perla M. Del Rio Estrada, Mauricio González-Navarro, Kenneth Lynn, Colin T. King, Sai Vemula, Kara Cox, Yanmin Wan, Qingsheng Li, Karam Mounzer, Jay Kostman, Ian Frank, Mirko Paiardini, Daria Hazuda, Gustavo Reyes-Terán, Douglas Richman, Bonnie Howell, Pablo Tebas, Javier Martinez-Picado, Vicente Planelles, Maria J. Buzon,* Michael R. Betts,* Luis J. Montaner*

*Corresponding author. Email: montaner{at}wistar.org (L.J.M.); mariajose.buzon{at}vhir.org (M.J.B.); betts{at}pennmedicine.upenn.edu (M.R.B.)

Published 18 April 2018, Sci. Transl. Med. 10, eaar6759 (2018)
DOI: 10.1126/scitranslmed.aar6759

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Representative example of the gating strategy used for analyses in Fig. 1.
  • Fig. S2. Percentage of cells expressing the activation markers HLA-DR, CD69, or CD25 on total CD4+ T cells, CD32+ CD4+ T cells, and CD32 CD4+ T cells.
  • Fig. S3. Phenotyping of CD32+ CD4+ cells from peripheral blood.
  • Fig. S4. Phenotyping of CD32+ CD4+ cells from tonsils.
  • Fig. S5. CD4+ CD32+ T cells in the LN of ART-suppressed, SIV-infected RMs are enriched in activation markers but not in SIV DNA.
  • Fig. S6. Lack of correlations between frequency of CD32+ of resting CD4+ T cells and HIV DNA and RNA loads.
  • Fig. S7. Correlations between frequency of CD32 CD4+ T cells and HIV DNA and RNA loads.
  • Fig. S8. Different HIV RNA and CD32a RNA pattern expression in LN samples from HIV-infected patients.
  • Table S1. Clinical data of patients included in Figs. 1 and 5.
  • Table S2. DNA quantity and number of sorted cells examined in Fig. 5C.
  • Table S3. Clinical data of patients included in Figs. 2, 3, and 6.
  • References (5153)

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Other Supplementary Material for this manuscript includes the following:

  • Table S4 (Microsoft Excel format). Primary data.