Supplementary Materials

Supplementary Material for:

The systemic response to surgery triggers the outgrowth of distant immune-controlled tumors in mouse models of dormancy

Jordan A. Krall, Ferenc Reinhardt, Oblaise A. Mercury, Diwakar R. Pattabiraman, Mary W. Brooks, Michael Dougan, Arthur W. Lambert, Brian Bierie, Hidde L. Ploegh, Stephanie K. Dougan, Robert A. Weinberg*

*Corresponding author. Email: weinberg{at}

Published 11 April 2018, Sci. Transl. Med. 10, eaan3464 (2018)
DOI: 10.1126/scitranslmed.aan3464

This PDF file includes:

  • Materials and Methods
  • Fig. S1. The outgrowth of D2A1-GFP tumors in Balb/c mice is restricted by a GFP-specific CD8+ T cell response.
  • Fig. S2. Surgical wounding triggers the outgrowth of tumor cells at distant anatomical sites.
  • Fig. S3. Meta-analyses demonstrate that surgical wounding promotes the outgrowth of distantly implanted tumor cells.
  • Fig. S4. Surgical wounding overcomes the effect of a tumor vaccine to promote the growth of distant B16 tumors.
  • Fig. S5. Surgical wounding triggers a systemic inflammatory response characterized by the mobilization of inflammatory myeloid cells into the circulation.
  • Fig. S6. Myeloid cells infiltrate D2A1-GFP tumors and promote tumor growth.
  • Fig. S7. NSAIDs alter the polarization of tumor-infiltrating macrophages.
  • Table S1. Components of flow cytometry antibody cocktails.
  • Reference (56)

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Other Supplementary Material for this manuscript includes the following:

  • Table S2 (Microsoft Excel format). Primary data.