Supplementary Materials

Supplementary Material for:

T cell–induced CSF1 promotes melanoma resistance to PD1 blockade

Natalie J. Neubert, Martina Schmittnaegel, Natacha Bordry, Sina Nassiri, Noémie Wald, Christophe Martignier, Laure Tillé, Krisztian Homicsko, William Damsky, Hélène Maby-El Hajjami, Irina Klaman, Esther Danenberg, Kalliopi Ioannidou, Lana Kandalaft, George Coukos, Sabine Hoves, Carola H. Ries, Silvia A. Fuertes Marraco, Periklis G. Foukas, Michele De Palma,* Daniel E. Speiser*

*Corresponding author. Email: michele.depalma{at}epfl.ch (M.D.P.); doc{at}dspeiser.ch (D.E.S.)

Published 11 April 2018, Sci. Transl. Med. 10, eaan3311 (2018)
DOI: 10.1126/scitranslmed.aan3311

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Selection of regions with high or low density of tumor-infiltrating CD8+ T cells.
  • Fig. S2. Correlations between genes expressed in the SKCM metastatic cohort.
  • Fig. S3. CSF1+ cell density in high and low CD8+ cell-infiltrated tumor regions.
  • Fig. S4. CSF1 expression by melanoma cell lines and CTLs.
  • Fig. S5. Absence of CSF1R on the surface of untreated or cytokine-treated melanoma cell lines.
  • Fig. S6. Gene expression in human melanoma cells exposed to MelanA-specific CTLs or IFNγ and TNFα.
  • Fig. S7. Expression of macrophage genes in a transcriptomic data set of human melanoma treated with anti-PD1 therapy.
  • Fig. S8. Impact of CTL and TAM infiltrates on melanoma prognosis.
  • Fig. S9. Concentration of CSF1 in cell culture medium conditioned by mouse melanoma cells.
  • Table S1. Melanoma patients, clinical parameters, and specimens analyzed in this study.
  • Table S2. Origin of the melanoma cell lines used in this study.
  • Table S3. List of melanoma-derived macrophage signature genes.
  • Table S4. A selection of currently active clinical trials of CSF1 or CSF1R blockade in combination with immune checkpoint blockade (ClinicalTrials.gov; 18 October 2017).
  • References (86109)

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S5 (Microsoft Excel format). Primary raw data shown in the figures.