Supplementary Materials

Supplementary Material for:

Commensal orthologs of the human autoantigen Ro60 as triggers of autoimmunity in lupus

Teri M. Greiling, Carina Dehner, Xinguo Chen, Kevin Hughes, Alonso J. Iñiguez, Marco Boccitto, Daniel Zegarra Ruiz, Stephen C. Renfroe, Silvio M. Vieira, William E. Ruff, Soyeong Sim, Christina Kriegel, Julia Glanternik, Xindi Chen, Michael Girardi, Patrick Degnan, Karen H. Costenbader, Andrew L. Goodman, Sandra L. Wolin,* Martin A. Kriegel*

*Corresponding author. Email: martin.kriegel{at}yale.edu (M.A.K.); sandra.wolin{at}nih.gov (S.L.W.)

Published 28 March 2018, Sci. Transl. Med. 10, eaan2306 (2018)
DOI: 10.1126/scitranslmed.aan2306

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Sequence alignment of full-length hRo60 and selected commensal orthologs.
  • Fig. S2. Coimmunoprecipitation of lupus study subject sera confirmed anti-Ro60 antibody status.
  • Fig. S3. α-Diversity represented by box plots of the Shannon-Weiner diversity index.
  • Fig. S4. SCLE skin eruption.
  • Fig. S5. TT-reactive CD4+ T cell clone from a healthy donor generated by a CD4 T cell library assay.
  • Fig. S6. Ro60-negative SLE patient CD4+ T cells lack reactivity to hRo60 protein.
  • Fig. S7. Cytokine concentrations (pg/ml) of supernatants from the cross-reactive T cell clone from Fig. 5 measured using a bead-based immunoassay.
  • Fig. S8. Anti-Ro60 antibody status of Harvard cohort lupus subjects.
  • Fig. S9. YrlA RNA is not detected in immunoprecipitates from B. theta using human lupus sera.
  • Fig. S10. B. theta monocolonization of GF mice induces gut and systemic immune changes.
  • Fig. S11. Schematic of proposed mechanism of how Ro60 bacteria trigger and sustain autoimmunity.
  • Table S1. Commensal bacterial Ro60 orthologs identified by in silico methods.
  • Table S2. Lupus study subject clinical data.
  • Table S3. Healthy control study subject clinical data.
  • Table S4. Efficiency and specificity of bacterial Ro60 qPCR primers.
  • References (6377)

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S5 (Microsoft Excel format). Primary data.