Supplementary Materials

Supplementary Material for:

An OX40/OX40L interaction directs successful immunity to hepatitis B virus

Jean Publicover, Anuj Gaggar, Jillian M. Jespersen, Ugur Halac, Audra J. Johnson, Amanda Goodsell, Lia Avanesyan, Stephen L. Nishimura, Meghan Holdorf, Keith G. Mansfield, Joyce Bousquet Judge, Arya Koshti, Michael Croft, Adil E. Wakil, Philip Rosenthal, Eric Pai, Stewart Cooper, Jody L. Baron*

*Corresponding author. Email: jody.baron{at}ucsf.edu

Published 21 March 2018, Sci. Transl. Med. 10, eaah5766 (2018)
DOI: 10.1126/scitranslmed.aah5766

This PDF file includes:

  • Fig. S1. Age-dependent expression of OX40L on hepatic APCs.
  • Fig. S2. OX40L mRNA expression in various tissues, strains, and liver-derived cell populations.
  • Fig. S3. Adult HBVEnvRag−/− mice have more hepatic CD4+ lymphocytes and more circulating and hepatic OX40+ CD4+ lymphocytes 8 days after adoptive transfer compared to young mice.
  • Fig. S4. Lack of OX40 expression on lymphocytes alters hepatitis, HBsAg clearance, HBsAb seroconversion, and T cell numbers but does not impair HBcAb seroconversion in HBVRplRag−/− mice or HBsAb seroconversion in response to the human HBV vaccine.
  • Fig. S5. Adoptive transfer of adult WT splenocytes into HBVEnvRag−/− adult mice treated with an OX40L blocking antibody abrogates biochemical hepatitis but does not alter long-term HBsAg clearance or HBsAb production.
  • Fig. S6. Young HBVtgRag−/− mice treated with the OX40 agonist show increased absolute numbers of CD4+ T cells and TFH cells eight days after adoptive transfer of splenocytes.
  • Fig. S7. Infants do not have decreased expression of transcripts associated with monocyte and macrophage populations relative to adults.
  • Fig. S8. Patients with AHB who clear the virus have increased percentages of PBMC-derived CD4+ T cells, and a greater percentage of these CD4+ T cells express OX40.
  • Table S1. Peripheral expression of HBsAg at given times after adoptive transfer.
  • Table S2. Identified 15-mer HBV envelope peptide sequences for ELISpot containing dominant and subdominant epitopes.

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Other Supplementary Material for this manuscript includes the following:

  • Table S3 (Microsoft Excel format). Primary data.