Supplementary Materials

Supplementary Material for:

Integrated molecular imaging reveals tissue heterogeneity driving host-pathogen interactions

James E. Cassat, Jessica L. Moore, Kevin J. Wilson, Zach Stark, Boone M. Prentice, Raf Van de Plas, William J. Perry, Yaofang Zhang, John Virostko, Daniel C. Colvin, Kristie L. Rose, Audra M. Judd, Michelle L. Reyzer, Jeffrey M. Spraggins, Caroline M. Grunenwald, John C. Gore, Richard M. Caprioli, Eric P. Skaar*

*Corresponding author. Email: eric.skaar{at}vanderbilt.edu

Published 14 March 2018, Sci. Transl. Med. 10, eaan6361 (2018)
DOI: 10.1126/scitranslmed.aan6361

This PDF file includes:

  • Fig. S1. Iron- and manganese-responsive fluorescent bacterial reporters coupled with LA-ICP-MS reveal lesional dynamics during invasive infection.
  • Fig. S2. MALDI IMS reveals the bacterial niche and lesional dynamics in abscessed tissues during invasive S. aureus infection.
  • Legends for movies S1 to S10

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Full MRI image volume.
  • Movie S2 (.mp4 format). Co-registration of MRI to 3D BLI.
  • Movie S3 (.mp4 format). Co-registration of blockface digital imaging to 3D BLI.
  • Movie S4 (.mov format). Co-registration of blockface digital imaging to LA-ICPMS imaging of calcium, copper, iron, magnesium, manganese, phosphorous, and zinc.
  • Movie S5 (.mp4 format). Co-registration of blockface digital imaging to 3D BLI and LA-ICP-MS imaging of iron.
  • Movie S6 (.mp4 format). Co-registration of MRI to MALDI IMS signal for the S100A8 component of calprotectin (m/z = 10,164).
  • Movie S7 (.mp4 format). Co-registration of blockface digital imaging to MALDI IMS signal for the S100A8 component of calprotectin (m/z = 10,164).
  • Movie S8 (.mp4 format). Co-registration of blockface digital imaging to MALDI IMS signal for the S100A8 component of calprotectin (m/z = 10,164).
  • Movie S9 (.mov format). Co-registration of blockface digital imaging to MALDI IMS signal for the S100A8 component of calprotectin (m/z = 10,164) and LA-ICP-MS imaging of calcium.
  • Movie S10 (.mp4 format). Co-registration of blockface digital imaging to MALDI IMS signals for the S100A8 component of calprotectin (m/z = 10,164) and S. aureus δ-hemolysin (m/z = 3006).