Supplementary Materials

Supplementary Material for:

The Fas/Fap-1/Cav-1 complex regulates IL-1RA secretion in mesenchymal stem cells to accelerate wound healing

Xiaoxing Kou, Xingtian Xu, Chider Chen, Maria Laura Sanmillan, Tao Cai, Yanheng Zhou, Claudio Giraudo, Anh Le, Songtao Shi*

*Corresponding author. Email: songtaos{at}upenn.edu

Published 14 March 2018, Sci. Transl. Med. 10, eaai8524 (2018)
DOI: 10.1126/scitranslmed.aai8524

This PDF file includes:

  • Materials and methods
  • Fig. S1. GMSCs secrete higher amounts of sEVs and cytokines.
  • Fig. S2. Fas controls IL-1RA–sEV secretion in murine SMSCs.
  • Fig. S3. Fas/Fap-1 binds with Cav-1 to control SNAP25/VAMP5-mediated IL-1RA release in murine MSCs.
  • Fig. S4. TNF-α promotes sEV and IL-1RA release in murine MSCs.
  • Fig. S5. Histomorphology of IL-1RA in wound healing in mice.
  • Fig. S6. sEVs containing IL-1RA ameliorate delayed wound healing in diabetic mice.
  • Fig. S7. Histomorphology of Fas in wound healing in mice.
  • Fig. S8. Schematic drawing of Fas/Fap-1/Cav-1–controlled IL-1RA–sEV secretion in MSCs.
  • Fig. S9. Characterization of BMMSCs, GMSCs, and SMSCs.
  • Legends for movies S1 to S3

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Individual subject-level data.
  • Movie S1 (.mov format). GMSCs secrete IL-1RA–positive exosome-like EVs.
  • Movie S2 (.mov format). Exocytotic fusions of IL-1RA–positive vesicles in living GMSCs.
  • Movie S3 (.mov format). TNF-α–activated GMSCs release IL-1RA–positive exosome-like EVs.
  • Database S1 (.pdf format). Western blotting films corresponding to Figs. 1 to 4.