Supplementary Materials

Supplementary Material for:

T follicular helper–like cells contribute to skin fibrosis

Devon K. Taylor,* Nanette Mittereder, Ellen Kuta, Tracy Delaney, Timothy Burwell, Karma Dacosta, Weiguang Zhao, Lily I. Cheng, Charles Brown, Anmarie Boutrin, Xiang Guo, Wendy I. White, Jie Zhu, Huifang Dong, Michael A. Bowen, Jia Lin, Changshou Gao, Li Yu, Madhu Ramaswamy, Marie-Claude Gaudreau, Rob Woods, Ronald Herbst, Gianluca Carlesso*

*Corresponding author. Email: taylord{at}medimmune.com (D.K.T.); carlessog{at}medimmune.com(G.C.)

Published 7 March 2018, Sci. Transl. Med. 10, eaaf5307 (2018)
DOI: 10.1126/scitranslmed.aaf5307

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Skin transcription factor profiling and correlational analysis of infiltrating T cells and molecules related to disease activity.
  • Fig. S2. Evaluation of cellular subsets in the fibrotic skin of mice with GVHD-induced skin fibrosis.
  • Fig. S3. Anti–ICOS-aFuc MAb elicits efficient depletion of ICOS-expressing T cells.
  • Fig. S4. Anti-ICOS treatment blocks B cell functional response.
  • Fig. S5. Skin-infiltrating T cells express both proinflammatory and profibrotic cytokines.
  • Fig. S6. Anti-ICOS treatment reduced the systemic levels of molecules involved in lymphocyte function, migration, and fibrosis.
  • Table S1. Correlative comparison of mRNA transcript signature for TH1, TH2, and TFH phenotypic molecules in fibrotic skin of patients with SSc.
  • Table S2. Summary of binding affinity for parental and therapeutic antibody constructs.
  • Table S3. Designated probes used for screening human SSc and murine GVHD-SSc genes.
  • References (6264)

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Other Supplementary Material for this manuscript includes the following:

  • Table S4 (Microsoft Excel format). Primary data.