Supplementary Materials

Supplementary Material for:

HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing

Steven A. Yukl,* Philipp Kaiser, Peggy Kim, Sushama Telwatte, Sunil K. Joshi, Mai Vu, Harry Lampiris, Joseph K. Wong

*Corresponding author. Email: steven.yukl{at}ucsf.edu

Published 28 February 2018, Sci. Transl. Med. 10, eaap9927 (2018)
DOI: 10.1126/scitranslmed.aap9927

This PDF file includes:

  • Fig. S1. HIV RNA assays to investigate the mechanisms that regulate transcription.
  • Fig. S2. Assay validation and performance characteristics.
  • Fig. S3. Differences in quantities of HIV DNA sequence regions are significant but small relative to observed differences in the corresponding HIV RNAs.
  • Fig. S4. Distribution of HIV transcripts and blocks in serial, replicate dilutions of unstimulated and activated CD4+ T cells.
  • Fig. S5. Effect of ex vivo treatment with latency-reversing agents on different HIV transcripts (in copies per microgram of RNA).
  • Fig. S6. Effect of latency-reversing agents by individual agent.
  • Table S2. Clinical characteristics of the study participants.
  • Table S3. Primers and probes.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Data in tabular form.