Supplementary Materials

Supplementary Material for:

Rapid detection of Mycobacterium tuberculosis in sputum with a solvatochromic trehalose probe

Mireille Kamariza, Peyton Shieh, Christopher S. Ealand, Julian S. Peters, Brian Chu, Frances P. Rodriguez-Rivera, Mohammed R. Babu Sait, William V. Treuren, Neil Martinson, Rainer Kalscheuer, Bavesh D. Kana, Carolyn R. Bertozzi*

*Corresponding author. Email: bertozzi{at}stanford.edu

Published 28 February 2018, Sci. Transl. Med. 10, eaam6310 (2018)
DOI: 10.1126/scitranslmed.aam6310

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Synthesis of DMN-Tre and DMN-Glc.
  • Fig. S2. DMN-Tre labeling is concentration-dependent.
  • Fig. S3. Optimization of DMN-Tre detection.
  • Fig. S4. DMN-Tre labeling is specific to bacteria bearing mycomembranes.
  • Fig. S5. DMN-Tre labeling does not primarily rely on the intracellular trehalose metabolic pathway.
  • Fig. S6. Msmeg ΔMSMEG:6396-6399 gene deletion mutant exhibits reduced labeling with DMN-Tre.
  • Fig. S7. DMN-Tre is incorporated into trehalose monomycolate in the mycobacterial outer membrane.
  • Fig. S8. Loss of lipid tails from DMN-Tre–corynemonomycolates is detected by tandem mass spectrometry.
  • Fig. S9. Drug cocktail kills Msmeg cells in vitro.
  • Fig. S10. DMN-Tre time course labeling of H37Rv axenic cultures.
  • Fig. S11. Drug cocktail kills Mtb cells and inhibits DMN-Tre incorporation into the mycomembrane.
  • Fig. S12. Rifampin-treated Mtb cells do not label with DMN-Tre, even in the presence of culture filtrates from live Mtb cells.
  • Fig. S13. DMN-Tre detects Mtb cells in sputum samples comparably to auramine staining.
  • Table S1. List of strains with Ag85 homology.
  • Reference (46)

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Other Supplementary Material for this manuscript includes the following:

  • Appendix S1 (.pdf format). 1H and 13C nuclear magnetic resonance spectra.