Supplementary Materials

Supplementary Material for:

Eosinophil depletion suppresses radiation-induced small intestinal fibrosis

Naoki Takemura, Yosuke Kurashima, Yuki Mori, Kazuki Okada, Takayuki Ogino, Hideki Osawa, Hirosih Matsuno, Lamichhane Aayam, Satoshi Kaneto, Eun Jeong Park, Shintaro Sato, Kouta Matsunaga, Yusuke Tamura, Yasuo Ouchi, Yutaro Kumagai, Daichi Kobayashi, Yutaka Suzuki, Yoshichika Yoshioka, Junichi Nishimura, Masaki Mori, Ken J. Ishii, Mark E. Rothenberg, Hiroshi Kiyono, Shizuo Akira, Satoshi Uematsu*

*Corresponding author. Email: suematsu{at}chiba-u.jp

Published 21 February 2018, Sci. Transl. Med. 10, eaan0333 (2018)
DOI: 10.1126/scitranslmed.aan0333

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Histology of human RIF.
  • Fig. S2. Detection of collagen fibers in mouse small intestine after abdominal irradiation.
  • Fig. S3. Migration of α-SMA+ cells from the pericryptal area after abdominal irradiation.
  • Fig. S4. Detection of T cells in mouse small intestine after abdominal irradiation.
  • Fig. S5. Eosinophil recruitment to the SM in Rag2−/− mice after abdominal irradiation.
  • Fig. S6. Mouse BM-derived eosinophils (BMEos).
  • Fig. S7. Anti-CCR3 Ab treatment ameliorates RIF.
  • Fig. S8. RIF in P2x7r−/− mice after abdominal irradiation.
  • Fig. S9. ATP receptor expression of intestinal α-SMA+ stromal cells.
  • Fig. S10. Suppression of RIF by blocking ATP-mediated activation of P2X receptors.
  • Fig. S11. Chemokine protein array analysis of intestinal α-SMA+ cells.
  • Fig. S12. RIF in Il33−/− mice after abdominal irradiation.
  • Fig. S13. RIF in Rag2−/−γc−/− mice after abdominal irradiation.
  • Fig. S14. In vitro analyses of anti–IL-5Rα activities of cmIL5Ra1b12.
  • Fig. S15. Eosinophil depletion by repeated treatment with cmIL5Ra1b12.
  • Fig. S16. Hydroxyproline levels of the small intestines of cmIL5Ra1b12-treated WT mice.
  • Table S1. Gene expression of intestinal eosinophils after GM-CSF stimulation.
  • References (4450)

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Other Supplementary Material for this manuscript includes the following:

  • Table S2 (Microsoft Excel format). Primary data.