Supplementary Materials

Supplementary Material for:

Microhemorrhage-associated tissue iron enhances the risk for Aspergillus fumigatus invasion in a mouse model of airway transplantation

Joe L. Hsu, Olga V. Manouvakhova, Karl V. Clemons, Mohammed Inayathullah, Allen B. Tu, Raymond A. Sobel, Amy Tian, Hasan Nazik, Venkata R. Pothineni, Shravani Pasupneti, Xinguo Jiang, Gundeep S. Dhillon, Harmeet Bedi, Jayakumar Rajadas, Hubertus Haas, Laure Aurelian, David A. Stevens, Mark R. Nicolls*

*Corresponding author. Email: mnicolls{at}

Published 21 February 2018, Sci. Transl. Med. 10, eaag2616 (2018)
DOI: 10.1126/scitranslmed.aag2616

This PDF file includes:

  • Materials and Methods
  • Table S1. RT-qPCR genes and primers.
  • Fig. S1. Iron content, as measured by ICP-MS, in day 12 syntransplants and allotransplants.
  • Fig. S2. RT-qPCR of host iron metabolism genes in day 12 syntransplants and allotransplants.
  • Fig. S3. Schematic of culture plate for growth morphology and positive tropism studies.
  • Fig. S4. Culture plates showing positive tropism.
  • Fig. S5. Experimental design of animal studies.
  • Fig. S6. Semiquantitative histopathological scale for grading fungal invasion and burden.
  • Fig. S7. Fungal burden by iron groups in the orthotopic tracheal transplantation model.
  • Fig. S8. Increased fungal invasion in allotransplants treated with FeSO4 topical solution.
  • Fig. S9. Alloimmune-mediated microvascular ischemia and iron overload do not decrease immune effector cell numbers in the orthotopic tracheal transplantation model.
  • Fig. S10. Schematic diagram illustrating how acute allograft rejection induces transplant iron overload, promoting an invasive A. fumigatus phenotype.

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