Supplementary Materials

Supplementary Material for:

Microenvironmental regulation of the IL-23R/IL-23 axis overrides chronic lymphocytic leukemia indolence

Giovanna Cutrona,* Claudio Tripodo, Serena Matis, Anna Grazia Recchia, Carlotta Massucco, Marina Fabbi, Monica Colombo, Laura Emionite, Sabina Sangaletti, Alessandro Gulino, Daniele Reverberi, Rosanna Massara, Simona Boccardo, Daniela de Totero, Sandra Salvi, Michele Cilli, Mariavaleria Pellicanò, Martina Manzoni, Sonia Fabris, Irma Airoldi, Francesca Valdora, Silvano Ferrini, Massimo Gentile, Ernesto Vigna, Sabrina Bossio, Laura De Stefano, Angela Palummo, Giovanni Iaquinta, Martina Cardillo, Simonetta Zupo, Giannamaria Cerruti, Adalberto Ibatici, Antonino Neri, Franco Fais, Manlio Ferrarini, Fortunato Morabito

*Corresponding author. Email: giovanna.cutrona{at}hsanmartino.it

Published 14 February 2018, Sci. Transl. Med. 10, eaal1571 (2018)
DOI: 10.1126/scitranslmed.aal1571

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Gating strategy to analyze IL-23R complex induction after activation by CD40L-expressing fibroblasts.
  • Fig. S2. IL-12Rβ1 mRNA production after CLL cell activation by CD40L-expressing fibroblasts.
  • Fig. S3. Expression of a complete IL-23R by CLL cells after depletion of IL-23R–positive cells and coculture with autologous activated T cells.
  • Fig. S4. Demonstration of intracytoplasmic IL-23 chains in CLL cells.
  • Fig. S5. HS5 stromal cells do not express CD40L.
  • Fig. S6. Enhanced IL-23R complex expression by coculture of PBMC with NLC.
  • Fig. S7. IL-23R complex expression by CLL cells cocultured with stromal cells or NLCs.
  • Fig. S8. Relationship between the BCR signaling pathway and IL-23R complex expression in CLL cells.
  • Fig. S9. Silencing of IL-23A or IL-23R genes by siRNA.
  • Fig. S10. STAT3 phosphorylation induced by CLL cell exposure to IL-23.
  • Fig. S11. NSG mice engrafted with CLL cells.
  • Fig. S12. Minimal residual disease detection in mice tissues after treatment with αIL-23p19.
  • Fig. S13. Anti–IL-23p19 mAb does not induce antibody-dependent cytotoxicity.
  • Fig. S14. Schematic representation of the IL-23R complex/IL-23 axis in CLL.
  • Table S1. Relationship between IL-23R chain expression and prognostic parameters.
  • Table S2. Univariate and multivariate bootstrapping validated Cox regression analysis of TTFT.
  • Table S3. Features of the CLL cases used in coculture experiments with CD40L-expressing fibroblasts (CD40L-TC).
  • Table S4. Summary of the features of all CLL cases whose cells were used for xenograft tests.
  • References (5053)

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Other Supplementary Material for this manuscript includes the following:

  • Table S5 (Microsoft Excel format). Primary data.