Supplementary Materials

Supplementary Material for:

Histone deacetylase activity governs diastolic dysfunction through a nongenomic mechanism

Mark Y. Jeong, Ying H. Lin, Sara A. Wennersten, Kimberly M. Demos-Davies, Maria A. Cavasin, Jennifer H. Mahaffey, Valmen Monzani, Chandrasekhar Saripalli, Paolo Mascagni, T. Brett Reece, Amrut V. Ambardekar, Henk L. Granzier, Charles A. Dinarello, Timothy A. McKinsey*

*Corresponding author. Email: timothy.mckinsey{at}ucdenver.edu

Published 7 February 2018, Sci. Transl. Med. 10, eaao0144 (2018)
DOI: 10.1126/scitranslmed.aao0144

This PDF file includes:

  • Materials and Methods
  • Fig. S1. DSS rats fed a 4% NaCl–containing diet develop diastolic dysfunction with preserved EF.
  • Fig. S2. Treatment of ARVMs in culture with ITF2357 led to acceleration of myocyte relaxation kinetics without affecting contractility.
  • Fig. S3. HDAC inhibition does not affect HS diet–induced increases in β-MyHC expression or function.
  • Fig. S4. Myofibril calcium sensitivity is unaffected by HS feeding or ITF2357 treatment of DSS rats.
  • Fig. S5. Titin expression and function are unaffected by HS feeding or ITF2357 treatment of DSS rats.
  • Fig. S6. Myofibrillar protein phosphorylation and expression are unaffected by HS feeding or ITF2357 treatment of DSS rats.
  • Fig. S7. Site-specific phosphorylation of myofibrillar protein and myofibrillar protein expression are unaffected by HS feeding or ITF2357 treatment of DSS rats.
  • Fig. S8. HDAC2 co-purifies with cardiac myofibrils.
  • Fig. S9. Ex vivo acetylation/deacetylation does not alter myofibril contraction.
  • Fig. S10. Anti–acetyl-lysine immunoblotting of cardiac myofibrils from DSS rats.
  • Fig. S11. A model for HDAC inhibitor–mediated improvement in diastolic function and treatment of HFpEF.
  • Table S1. Hematological profiles of DSS rats.
  • Table S2. LV hemodynamic and echocardiographic parameters of DSS rats.
  • Table S3. Quantification of myocyte cross-sectional area and interstitial fibrosis in DSS rats.
  • Table S4. Echocardiographic parameters of aging mice.
  • Legend for table S5
  • Table S6. Characteristics of the RCM patients and nonfailing donor controls.
  • Table S7. Group sizes of animal models.

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Other Supplementary Material for this manuscript includes the following:

  • Table S5. Echocardiographic, hemodynamic, and hypertrophy parameters of aging mice. (Excel file format)