Supplementary Materials

Supplementary Material for:

Targeting p53-dependent stem cell loss for intestinal chemoprotection

Brian J. Leibowitz, Liheng Yang, Liang Wei, Monica E. Buchanan, Madani Rachid, Robert A. Parise, Jan H. Beumer, Julie L. Eiseman, Robert E. Schoen, Lin Zhang, Jian Yu*

*Corresponding author. Email: yuj2{at}upmc.edu

Published 7 February 2018, Sci. Transl. Med. 10, eaam7610 (2018)
DOI: 10.1126/scitranslmed.aam7610

This PDF file includes:

  • Materials and Methods
  • Fig. S1. PUMA KO inhibits CPT-11–induced crypt apoptosis.
  • Fig. S2. CPT-11 causes dose-dependent lethality in mice.
  • Fig. S3. A small-molecule PUMAi does not protect colon cancer cells against CPT-11–induced apoptosis.
  • Fig. S4. PUMAi inhibits CPT-11–induced CBC apoptosis.
  • Fig. S5. PUMAi protects against chemotherapy- and radiation-induced lethality.
  • Fig. S6. Targeting PUMA does not compromise tumor response to CPT-11.
  • Fig. S7. 5-FU–induced LGR5+ cell apoptosis is PUMA-dependent.
  • Fig. S8. Puma KO and PUMAi suppress CPT-11–induced expression of WNT and NOTCH targets.
  • Table S1. Treatment-associated lethality in LLC tumor experiments.
  • Table S2. Mouse-specific primers used for real-time reverse transcription polymerase chain reaction.
  • Table S3. Human-specific primers used for real-time reverse transcription polymerase chain reaction.
  • References (58, 59)

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