Supplementary Materials

Supplementary Material for:

Integrin α4β7 expression on peripheral blood CD4+ T cells predicts HIV acquisition and disease progression outcomes

Aida Sivro, Alexandra Schuetz, Daniel Sheward, Vineet Joag, Sergey Yegorov, Lenine J. Liebenberg, Nonhlanhla Yende-Zuma, Andrew Stalker, Ruth S. Mwatelah, Philippe Selhorst, Nigel Garrett, Natasha Samsunder, Anisha Balgobin, Fatima Nawaz, Claudia Cicala, James Arthos, Anthony S. Fauci, Aggrey Omu Anzala, Joshua Kimani, Bernard S. Bagaya, Noah Kiwanuka, Carolyn Williamson, Rupert Kaul, Jo-Ann S. Passmore, Nittaya Phanuphak, Jintanat Ananworanich, Aftab Ansari, Quarraisha Abdool Karim, Salim S. Abdool Karim, Lyle R. McKinnon,* on behalf of the CAPRISA004 and RV254 study groups

*Corresponding author. Email: lyle.mckinnon{at}umanitoba.ca

Published 24 January 2018, Sci. Transl. Med. 10, eaam6354 (2018)
DOI: 10.1126/scitranslmed.aam6354

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Effect of preinfection β7 int CD45RA+CD4+ and β7 Neg CD45RACD4+ T cell frequency on HIV acquisition.
  • Fig. S2. Phenotype of β7 Hi CD45RACD4+, β7int CD45RA+CD4+, and β7Neg CD45RACD4+ T cells.
  • Fig. S3. Effect of preinfection α4β7 + CD4+ T cell frequency in the blood on SIV acquisition in NHPs exposed to weekly intravaginal challenges with SIVmac251.
  • Fig. S4. Effect of preinfection β7 int CD45RA+CD4+ and β7 Neg CD45RACD4+ T cell frequency on disease progression in patients that became infected in CAPRISA 004/002 study.
  • Fig. S5. Gating strategy used for the analysis of frozen PBMCs from the CAPRISA 004 study.
  • Fig. S6. Effect of preinfection α4β7 + CD4+ T cell frequency in the gut and the blood on disease progression in RMs after intravenous injection with SIVmac239.
  • Table S1. Characteristics of the Ugandan and Kenyan study populations.
  • Table S2. Cohort characteristics (RV254 study).
  • Table S3. Cohort characteristics (FSW cohort; Nairobi, Kenya).
  • Table S4. Analysis of HIV acquisition and integrin β7Hi CD4+ T cells using conditional logistic regression in CAPRISA 004 (n = 165), in FSW cohort from Nairobi (n = 41), and in the combined cohort analysis (n = 206).
  • Table S5. Correlation of other immunological markers on bulk CD4+ T cells and CD4+ T cell decline of <500 cells/μl in the CAPRISA 004 study.
  • Table S6. Effect of preinfection integrin β7Hi CD4+ T cell frequency on postinfection plasma I-FABP and sCD14 expression using linear mixed model analysis adjusting for VL and CD4/CD8 ratio.
  • References (4448)

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Other Supplementary Material for this manuscript includes the following:

  • Table S7. Primary data (provided as an Excel file).